Dissertation > Excellent graduate degree dissertation topics show
Preparation of Nanometer Propolis and the Effects on Decrease Lipoidemia and Periodontitis of SD Rats
Author: DingHuangJie
Tutor: ZengZhiJiang
School: Jiangxi Agricultural University,
Course: Cultivation
Keywords: Nanometer propolis Decrease Lipoidemia Anti-oxidation periodontitis
CLC: R285.5
Type: Master's thesis
Year: 2012
Downloads: 8
Quote: 0
Read: Download Dissertation
Abstract
|
This experiment was conducted with nanometer propolis:in order to know the preparation of nanometer propolis and the effects on rats. The experiment was divided into two groups:one was the preparation method of nanometer propolis and the effects on Decrease Lipoidemia and Anti-oxidation of rats;the other was the effect of nanometer propolis on experimental periodontitis rats.Nanometer propolis was prepared with ethyl acetate as oil phase, polysorbate-80and Span-80as surfactant. The particle size is around270nm, and concentration is9.56mg/ml.Healthy male SD rats were fed with high-lipid pellet feed for5days, then blood from eyepit vein was collected to detect the levels of serum TC, TG and HDL-C. The rats were randomized into4groups. Different feeds for each group were arranged as follows: the control group (without propolis), the high dose group(800mg/kg BW), the middle dose group(400mg/kg BW)and the low dose group(200mg/kg BW). Then they were fed with nanometer propolis and high-lipid pellet feed in the meantime for45d. The blood was collected on the45th day after fed with nanometer propolis and the levels of serum TC, TG and HDL-C were detected.Experiment2:sixty of5-weeks old teeth healthy rats(200g±20g, half of male and female), were randomly allotted to6groups of10rats each.They were control group, model control group, KouTai group, the high dose group(800mg/mL), the middle dose group(400mg/mL)and the low dose group(200mg/mL). half of male and female. The blood was collected and the blood index of periodontitis female and male rats, hormone of periodontitis female and male rats, RBC hemolyses of periodontitis rats were detected. The results showed that:1.Nanometer propolis was prepared with ethyl acetate as oil phase, polysorbate-80and Span-80as surfactant. The particle size is around270nm, and concentration is9.56mg/ml.2.The weight of rats in high dose group decreased significantly,but There are no effects on TC,TG and HDL-C;RBC hemolytic degree in trial group decreased subsequently,but there are no significant difference. 3. Effects on blood index of periodontitis rats:for female, white blood cell (WBC) and inflammatory cell in middle dose group decreased significantly, there were significant differences of WBC on the middle-dose group that compared with model control group(p<0.05).For male, there were significant differences of lymphocyte on the middle-dose group that compared with model control group(p<0.05).4.Effects on hormone of periodontitis female rats:the levels of estradiol and progesterone in serum had no notable regular change after fed with NP for periodontitis female rats, the levels of progesterone decreased significantly on experimental group after fed with NP that compared with model control group(p<0.05).5.Effects on RBC hemolyses of periodontitis rats:there were no significant differences between control group, model control group, KouTai group, the high dose group,the middle dose group and the low dose group.
|
Related Dissertations
- Studies on Extraction, Isolation, Purification and Antioxidation Activity of Flavonoids from the Fruits of Avicennia Marina,S793.9
- Research of Glycosaminoglycan from Perna Virids Effect on Blood Lipid,R285.5
- Study on Extraction,purification and Antioxidant Activity of Wheat Germ Polysaccharides,TS210.1
- The Anti-oxidation Activity and Microencapsulation of the Extract from Eucommia Ulmoide Oliver Leaves,R285.5
- Study on Radix Pueraria Isoflavones Extraction by Fermentation and Its Antioxidation,TQ920.6
- Effects of in Ovo Equol Injection on Posthatch Growth and Meat Quality of Broilers and Mechanism Research,S831
- Study on Expression of MMP-8 on Experimental Periapial Periodontitis is Infected by Enterococcus Faecalis,R781.34
- The Study on Near Infrared Transmittance Spectroscopy and Analysis on Antioxidant Quality in Panicum Miliaceum L.,S516
- Differentiation Capacity of Periodontal Ligament Stem Cells of Chronic Periodontitis Patients with and Without Type 2 Diabetes Mellitus,R587.1
- Chondroitin sulfate and antioxidant effects on lipid metabolism in rats Alcoholism,R575
- Study on Extraction, Purification and Antioxidant Activities of Total Flavonoids from Peanut Shell,TQ461
- Research of Microstructure, Properties and Rare Earth Modification of Hot-dipped Aluminum on Carbon Steel,TG174.4
- Effect of Fermentation on Purple Potato Pigment and Development of Purple Potato Wine and Its Antioxidant Activity Evaluation,TS262
- Research of Interleukin-1 Genotype and Its Assocation, Prvotella Intermedia between Chronic Periodontitis and Coronary Heart Disease,R781.4
- Association of Tumor Necrosis Factor A Gene Polymorphism, Porphyromonas Gingivalis with Chronic Periodontitis and Coronary Heart Disease,R781.4
- Effect of Radix Hedysari and Its Compound on Performance, Antioxidation and Immune Function of Broilers,S831.5
- The Effects of Phytogenic Feed Additive on Growth Performance, Antioxidant Capability and Carcass Characteristics in Weanling Piglets and Finishing Pigs,S828.5
- Studies on Physiological Mechanisms of Salt Tolerance Improved by Selenium in Tomato (Lycopersicon Esculentum Mill),S641.2
- Extraction and Isolation of Chemical Composition from Viola Tianshanica Maxim and Their Antioxidantive Activities,S567.239
- The Role of Basic Fibroblast Growth Factor and Carnosine on the Rabbit Corneal Endothelial Cells Preserved in Mid-term Preserveing Medium,R779.65
- Research Chlorogenic Acid of Honeysuckle on Extraction, Separation and Anti-oxidation,R284
CLC: > Medicine, health > Chinese Medicine > Of Pharmacy > Pharmacology > Chinese medicine Experimental Pharmacology
© 2012 www.DissertationTopic.Net Mobile
|