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Study on Development of the Digestive System in Yellow Catfish Pelteobagrus Fulvidraco Larvae

Author: GaoChao
Tutor: YangRuiBin
School: Huazhong Agricultural University
Course: Fishery resources
Keywords: Digestive tract Development Histological Histochemical Quantitative PCR Mucous cells Pepsinogen H ~ / K ~-ATP enzyme Trypsinogen Fish larvae Pelteobagrus
CLC: S917.4
Type: Master's thesis
Year: 2011
Downloads: 62
Quote: 0
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Abstract

Yellow catfish (Pelteobagrus fulvidraco) affiliated Siluriformes, Bagrid, catfish genus, its meat is delicious and loved by the people, and in recent years has been in the country to carry out large-scale farming, but large-scale seed rearing process The high mortality rate of fish larvae, seriously restricted Pelteobagrus farming development. In morphological research group of the yellow catfish fish larvae digestive system development, the preliminary study on the basis of the histological and ultrastructural aspects of the study further histological and histochemical AB-PAS staining method Pelteobagrus, fish larvae digestive tract mucus cell development, and at the same time using quantitative PCR and other molecular biology methods pepsinogen of the yellow catfish fish larvae digestive system development process, H / K-ATP the enzymes trypsin original gene expression the quantitative detection research, in order to grasp the tissues and organs of the structure and function of the process of improvement, rich yellow catfish early life history information, as well as developed for the large-scale larval rearing of reasonable food ration strategy to improve fish larvae breeding The survival rate to provide a theoretical basis. The main findings are as follows: 1, histological HE staining and histochemical AB-PAS staining, histological findings on the yellow catfish fish larvae digestive tract, 1-day-old larvae oropharyngeal cavity closed, the stomach was closed oval shape , Cell no clear differentiation, the intestinal tract as an empty straight pipe, is not formed by bending, the differentiation of intestinal cells is not obvious; AB-PAS staining mucus cells is not detected. The 3-day-old larvae opening visible taste buds in the mouth, esophagus differentiated goblet cells, stomach tissue quickly differentiation and development, connectivity and esophagus and intestinal composition can basically be divided into three parts of the gastric cardia, gastric body and gastric pylorus mucosal epithelium simple columnar epithelium differentiated gastric adenocarcinoma, gastric pits and goblet cells, gastric mucosal folds obvious intestinal bent mucosa appeared folds, intestinal epithelial monolayer columnar cells, the nucleus located in the central cell, differentiated goblet cells; gastrointestinal AB-PAS staining is weak. 5-day-old AB-PAS staining showed the oropharyngeal cavity esophagus memory in a small amount of mucus type II cells, more conducive to the food swallowing; gastric epithelial type I mucous cells, has a certain role in regulating the acidic environment of the stomach, and help digestion; intestinal distribution of a small amount of the mucus type III cells and of type IV mucus cells, indicating that further improve digestive function; 15-day-old the esophagus distribution with a small amount of of type III mucus cells; responsibility door part of 25-day-old yellow catfish stomach cloth with a lot of gastric adenocarcinoma, gastric body is relatively small, the lack of pyloric gastric glands, muscularis oblique, circular and outer longitudinal rows apparent differentiation. The results showed that the fish larvae digestive tract, Pelteobagrus organizations rapidly developing in the 3-day-old basic structure With the age increasing structure gradually complete, 25-day-old close to the histological structure of adult fish. Quantitative PCR method, yellow catfish fish larvae in the digestive system pepsinogen, H / K-ATP enzyme and trypsinogen genes for quantitative detection, found that pepsinogen gene trypsinogen gene and H / K-ATP enzyme in 1-day-old expression, but the expression of a small amount; pepsinogen gene expression rapid increase in 1-day-old -8 days old, day 8 -23 days old expression did not increase significantly, expression levels after 23 days increased significantly; trypsinogen gene expression trends similar to pepsinogen gene close to the expression of a rapid increase in 1-day-old -6 days old, 6-day-old -23 days old expression present in an amount of ups and downs, and slowly increasing 23 day old expression increased significantly; H / K-ATPase gene expression of basic linear growth trend. The results showed that the yellow catfish larvae out of the film, the stomach environment is gradually soured. Experimental results show that the yellow catfish larvae after hatching, the protein digestive function rapidly developing digestive function in 6-23 days old, but still in the improvement process. 3, according to the results of this study, I believe that the 6-23 day-old the yellow catfish fish larvae still in the developmental sensitive period of digestion is still perfect the proposed transfer bait time after the 23-day-old.

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CLC: > Agricultural Sciences > Aquaculture, fisheries > Aquatic basic science > Aquatic Biology > Aquatic Zoology
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