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With today's society, people's living standards continue to improve and increase, relative to other meat, the lamb with its low fat, low cholesterol, high protein, pollution and so more and more consumers alike. Intramuscular fat (Intramuscular fat, IMF) content is an important indicator of meat quality evaluation. Heart-type fatty acid binding protein (Heart Fat Acid Binding Protein, H-FABP) gene affecting intramuscular fat (IMF) precipitated major gene. H-FABP gene promotes cell uptake of fatty acids affect the IMF content. Therefore the H-FABP as a candidate gene to study its relationship with production traits of animal breeding and molecular marker-assisted selection has some theoretical significance and practical value. This study Chongming Island, Shanghai 98 Chong understand goats, 100 Chongming miscellaneous goats, 91 goats and 65 Xuhuai Guanzhong dairy goat four adult goat populations as test material, the use of direct sequencing method to find some H-FABP exon2 and intron2 sequence of SNPs, and using PCR-RFLP looking into the sequence polymorphisms SNPs analysis; using Soxhlet extraction method to determine the intramuscular fat (IMF) content; using SPSS software to analyze H-FABP polymorphisms and their relationship with the IMF content. H-FABP part exon2 and intron2 sequence exists a point mutation (A1017G) and a point deletion (G999-), established Hae Ⅱ-RFLP and Sau Ⅰ-RFLP method to analyze A1017G and G999-two SNPs. A1017G locus through Hae Ⅱ-RFLP, get BB, Bb, bb3 genotypes, BB genotype was the dominant genotype. The χ2 test, Chong understand goats, goats and Xuhuai Chongming miscellaneous goat three goat populations on the restriction sites are in Hardy-Weinberg non-equilibrium, three goat populations PIC lt; 0.25, are much lower degree state; Guanzhong dairy goat population was monomorphic, there is only one kind of BB genotype. G999-sites via Sau Ⅰ-RFLP, get DD, Dd, dd3 genotypes, DD genotype was the dominant genotype. The χ2 test, Chong understand goat populations on the restriction sites in the Hardy-Weinberg non-equilibrium state, groups PIC lt; 0.25, as low polymorphism; while Chongming miscellaneous goats, Xuhuai Guanzhong dairy goats and goats 3 goat populations are monomorphic, there is only one kind of DD genotype, showing severe skewness. H-FABP towel A1017G and G999-2 points, respectively, for the IMF Offices in content to a certain extent, the IMF content of each genotype relationships least squares means that Bb gt; bb gt; BB, dd gt; Dd gt; DD. H-FABP can therefore infer IMF content as a candidate gene for goats and goat meat with the performance of molecular markers.
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