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Objective: In this study, in vitro cell culture and molecular biology techniques, human nasopharyngeal carcinoma cell line 5-8F as the research object, d-δ-tocotrienols on nasopharyngeal carcinoma cell proliferation, cell cycle and apoptosis analysis d-δ-tocotrienols in nasopharyngeal carcinoma malignant phenotype of cell biology related molecules Bax, Bcl-2 expression, by exploring the d-δ-tocotrienols anti nasopharynx cancer biological activity of d-δ-tocotrienols application of anti-nasopharyngeal experimental data and provide preliminary experimental evidence. Methods: In vitro culture, the experimental observation group and the control group, the control group without d-δ-tocotrienols intervention, the observation group with 40,50,60 μmol / l of d-δ-tocotrienols intervention. (1), cell morphology detection: d-δ-tocotrienols intervention NPC 5-8F cells after 48h, the cells were observed under an inverted microscope morphology; treated cells using Hoechst33258 dye, set fluorescence microscope apoptosis. (2), d-δ-tocotrienols intervention NPC 5-8F cells after 48h. MTT assay 5-8F cells. (3), d-δ-tocotrienols intervention NPC 5-8F cells after 48h, cell apoptosis was detected by flow cytometry change. (4), d-δ-tocotrienols intervention NPC 5-8F cells after 48h, flow cytometry cell cycle changes. (5), d-δ-tocotrienols intervention NPC 5-8F cells after 48h, using Western-blot detection of apoptosis-related proteins Bax, Bcl-2 expression. Results: (1), d-δ-tocotrienols handle 5-8F cells after 48h, with increasing concentration, microscopic observed decrease in the number of adherent living cells, increase in the number of floating dead cells, nuclear fluorescence intensity ratio of stained cells increased, significant cell growth inhibition and apoptosis. (2), cell proliferation analysis revealed that the cell growth inhibition observed group were (46.03 ± 4.16)%, (67.68 ± 3.12)%, (95.05 ± 0.10)%, compared with the control group, the inhibition rate 0 Compare, p lt ; 0.01, the difference was significant. (3), apoptosis analysis showed that apoptosis rate of the observation group were (2.60 ± 0.30)%, (7.54 ± 1.01)%, (21.17 ± 0.81)%, compared with the control group, the apoptosis rate (0.83 ± 0.12 ) comparing, p lt; 0.01, the difference was significant. (4) the observation group, the percentage of S phase cells were (27.67 ± 1.67)%, (31.37 ± 1.30)%, (33.00 ± 0.70)%, compared with the control group, the percentage of S phase cells was (16.33 ± 0.15)% compared, p lt; 0.01, the difference was statistically significant, were arrested in S phase cells significantly. (5), compared with the control group, the observation group increased expression of Bax, Bcl-2 protein expression decreased in a dose-by patience. Conclusion: d-δ-tocotrienols inhibit 5-8F cell proliferation, induce apoptosis, cells in S phase arrest, and its mechanism may be related to induction of expression of Bax, Bcl-2 expression inhibition of apoptosis regulating genes , a potential anti-NPC biological activity.
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