|
Objective To investigate the effect of Ulinastatin combined with glycerol fructose on aquaporin-4 expression in rats after intracerebral hemorrhage.Methods 100 SD rats were randomly divided into control group(A),intracerebral hemorrhage group (B), Ulinastatin group(C),glycerol fructose and Ulinastatin group(D), In addition to A group,other groups were made of a model of cerebral hemorrhage,using autologous arterial blood into the caudate nucleus of the legal system and symptoms of neurological impairment was a successful model.The B models were established by stereotaxic injection of autologous artery blood into the caudate nucleus. C group was injected intravenously Ulinastatin 50000 u/kg, D Group in the tail vein injection of glycerol fructose 5ml/kg and ulinastatin50000 u/ kg, ICH group given 8ml/kg saline at the same time, each group after modeling, respectively,6h, Id,3d,5d and 7d were killed, each time point,5 rats each. Immunohistochemical expression of AQP4 observed, Evans blue method to measure the blood-brain barrier permeability, brain water content of wet and dry weight method that cerebral edema.Results Compared with A group, AQP4 began to increase at 6h,3d peak,7d still higher than normal, D group was significantly less than the B group (P<0.05); BBB permeability after 6h in cerebral hemorrhage started to be high,1～3d top, then gradually decreased, BBB permeability after intracerebral hemorrhage were higher after 6h,1～3d maximum, then gradually decreased, C group was lower than B group, but the D group was significantly lower than the C group (P<0.05); B Group brain water content began to increase 6 h,3 d after the peak slow down,7 d still higher than normal (P<0.05); D group water content at all time points were lower than the B group and the C group (P<0.05), the most significant reduction in 6h.Conclusions Expression by upregulating the APQ 4, increased permeability BBB participate in the formation of brain edema in cerebral hemorrhage, and an early treatment for dehydration of glycerol combined fructose Ulinastatin can inhibit this process.
|