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Neuroprotective Effects of Hypothermia and Ethanol in Rat with Transient Cerebral Ischemia

Author: WangFei
Tutor: HeZhongYi
School: Ningxia Medical University
Course: Human Anatomy and Embryology
Keywords: Cerebral ischemia Reperfusion injury Mild hypothermia Infarction Exercise behavior Alcohol
CLC: R741
Type: Master's thesis
Year: 2011
Downloads: 32
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Abstract

The purpose mild hypothermia brain protection role has been widely recognized. The goal of our study was to compare the systemic hypothermia in a rat model of cerebral ischemia, three different head mild hypothermia and vascular Guinea cryogenic brain protective effect of mild hypothermia way. Methods Sprague-Dawley rats to 64, with the line bolt in the right side of the brain is blocked arterial 3h manufacturing stroke model. Different sub-hypothermia (systemic hypothermia on cerebral ischemia in rats (n = 16) before reperfusion head mild hypothermia, vascular Guinea low temperature) or without intervention. Each randomly selected eight infarct volume of brain temperature, keeping another eight to 28d exercise behavior studies. Results of brain temperature, vascular Guinea hypothermia group within 5min significantly reduce the temperature of the ischemic region of the brain cortex temperature decreased from 37.2 ± 0.1 ℃ to 33.4 ± 0.4 ℃, striatum temperature decreased from 37.5 ± 0.2 ℃ 33.9 ± 0.4 ℃, and maintained 60min of mild hypothermia after reperfusion. Systemic hypothermia group to achieve a similar level of the low-temperature and it takes six times. Head mild hypothermia group reached similar low temperature level needed 15min time, but it will return to normal temperature within 5min. Although mild hypothermia can produce brain protective effect, but contrast the non-intervention group, 54.2 ± 1.9%, head mild hypothermia group, 37.1 ± 1.0%, and the systemic hypothermia group 29.1 ± 3.4% of the volume of infarction, vascular Guinea hypothermia group (infarct volume was 10.7 ± 2.6%) of the cerebral protective effect undoubtedly more significant (p lt; 0.001). Vascular Guinea hypothermia group results of 14d and 28d detection of movement behavior is more significant (p lt; 0.001). Conclusions with respect to the other two groups of mild hypothermia, vascular Guinea hypothermia on cerebral ischemia in rats can be more effective to reduce infarct volume and improve neurological function. The purpose of many studies have shown, the reference mild to moderate alcohol can reduce the risk of ischemic stroke, which shows that alcohol may have the effect of brain protection. The purpose of this study is to establish a rat model of cerebral ischemia demonstrated a protective effect of alcohol on the brain of acute ischemic stroke. The method of Sprague-Dawley rats to 77, with the line bolt in the right side of the brain is blocked arterial 2h manufacturing stroke model. Cerebral ischemia rats were randomly divided into groups: infarct volume measurement study of alcohol (0.5g/kg, 1.0g/kg, 1.5g/kg group) as well as the brain of the alcoholic mild hypothermia group protective effect; spectrophotometric count method for the determination of whether the combined use of alcohol and thrombolytic agents (rt-PA, UK) will increase cerebral hemorrhage. Research results infarct volume compared 0.5g/kg alcohol group 49.7 ± 2.8% infarct volume, 1.0g/kg alcohol group was 42.0 ± 1.1% of the infarct volume, infarct volume was 53.9 ± 2.3% of the control group to 1.5g/kg alcohol group (infarct volume was 29.2 ± 1.8%) and brain protection effect is most pronounced with (F (3,31) = 30.59, p lt; 0.001). The mild hypothermia alcohol combination group (26.5 ± 2.9%) reduced the extent of infarct volume from a numerical point of view. Cerebral hemorrhage study found that the combination of both alcohol with the thrombolytic agent (rt-PA, UK) and does not aggravate cerebral hemorrhage, and provide a basis for the feasibility of clinical applications. Conclusion Our study proved 1.5g/kg alcohol has a good brain protective effect on stroke rats with systemic hypothermia group. And when used in combination with thrombolytic drugs does not aggravate cerebral hemorrhage.

Neuroprotective Effects of Hypothermia and Ethanol in Rat with Transient Cerebral Ischemia

Abstract

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