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Effect of Heat Shock Protein 70 Induced by Alanyl Glutamine on Inhibiting Tubulointerstitial Fibrosis in Unilateral Ureteral Obstruction Rats

Author: ZhaoHaiBo
Tutor: LiXun
School: Guangzhou Medical College
Course: Department of Urology
Keywords: Alanyl - glutamine dipeptide Heat shock protein 70 Obstructive nephropathy Renal interstitial fibrosis Nuclear factor κb a- smooth muscle actin
CLC: R692
Type: Master's thesis
Year: 2011
Downloads: 13
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Abstract


【Objective】 alanyl acid - glutamine dipeptide (Ala-Gln) induction of heat shock protein 70 in rat obstructive nephropathy model and possible mechanisms of renal interstitial fibrosis (UUO) . 【Methods 】 20 Wistar rats were randomly divided into four groups : sham operation group , UUO model group , of GLN treatment group and QUE group , n = 5 . 1 d start from the establishment of the UUO model , of GLN treatment group and QUE 1.125g/kg given dose solution of Ala-Gln (Ala-Gln 0.9% NaCl solution dubbed in a concentration of 3% solution ) by intraperitoneal injection , sham group intraperitoneal injection of 0.9% NaC1 solution to give the appropriate dose and UUO model QUE group advance 6h intraperitoneal injection of a heat shock protein blockers given quercetin (quercetin) , the dose of 400mg/kg daily . 7 days after the rats were sacrificed . HE staining and Masson staining the case of renal interstitial fibrosis . Western blotting was used to detect the level of kidney HSP70 expression . Immunohistochemical staining renal NF -κB , the expression levels of a- SMA . [Results] The GLN treatment group Ala-Gln induced kidney -specific high expression of heat shock protein 70 ( HSP70 ) showed low expression of the other three groups of rats kidney HSP70 . Compared with UUO model group , QUE group , of GLN treatment group kidneys NF - κB , the level of expression of a- SMA significantly reduced tubular injury and renal interstitial fibrosis is reduced significantly the tubular damage percentage (26.2% ± 1.6 % vs 78.4 % ± 3.5 % , 80.2 % ± 3.3 % ) and renal interstitial damage score (1.32 ± 0.08 vs 3.67 ± 0.22,3.70 ± 0.20 ) , P lt; 0.05 ] . [Conclusion] Ala-Gln , delaying the process of interstitial fibrosis in rats with obstructive nephropathy induced HSP70 expression , its mechanism may be related to HSP70 down NF-κB, reduce the expression of a-SMA , inhibition of renal tubular epithelial cells - between mesenchymal transdifferentiation (TEMT) .

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CLC: > Medicine, health > Surgery > Urology ( urinary and reproductive system diseases) > Kidney disease
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