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Objective: To establish a biofilm of Staphylococcus aureus (Staphylococcus aureus AS) of the titanium plate surface (Biofilm BF) In vitro model; research in vitro gallic extracts (Galla Chinesis GC) of staphylococcus aureus biofilm on clearance of the titanium plate surface synergistic role. Methods: X387 type AS ( biofilm formation positive strains ) , the establishment of in vitro titanium plate surface BF model . Tube double dilution method Determination of the minimum inhibitory concentration of the test drug (minimal inhibitorycib concentration MIC) gallic extracts , clindamycin (Clmindamycin CM) each drug is used alone or in mutual combined effect on the titanium sheet surface BF CLSM respectively observe the intervention of different drugs 4h , 8h , 12h , 24h titanium plate surface of Staphylococcus aureus biofilm generated . CLSM along the Z axis at different positions scanned , its thickness is measured and the organizational structure . Results: According to the experiment , GC AS the MIC 32mg/ml CM AS the MIC for 2mg/ml . GC, CM inhibit and destroy the titanium plate surface BF . BBF by the red , green and yellow fluorescence observed under CLSM red fluorescence of dead bacteria , green living bacteria , the yellow phase superposition of dead bacteria and living bacteria . In 4, 8, 12, 24 h , GC , CM Group , GC CM group , NS group role , AS biofilm morphology , structure, there are significant differences . Titanium plate surface bacteria by the experimental group scattered distribution gradually becomes dense , the formation of the group , or flake structure , but not a typical biofilm structure . Titanium plate surface bacteria NS group consists of loose gradually intensive and thickening the more typical biofilm dimensional structure . Titanium plate surface at different time points , the use of specific fluorescent marker and CLSM observed experimental group AS thickness of the biofilm , and dead bacteria , and the number of viable bacteria , and calculate the viable percentage . The results showed that the experimental group titanium plate surface AS biofilm thickness compared with NS group was statistically significant (P lt; 0.05); viable cells percentage of NS group compared statistically significant ( P lt; 0.01 ) . Conclusion : gall extracts inhibition produced on the titanium sheet surface AS early biofilm formation process , and can reduce the biofilm activity , change the structure of the AS biofilm destruction of its structural integrity . Titanium plate surface AS biofilm cleanup was Galla chinensis extract in combination with clindamycin synergy.
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