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Inhibition of Berberine Hydrochloride on Drug Resistance Research and Propionibacterium on Resistant Strains

Author: ZengXianYu
Tutor: YangZhiBo
School: Hunan University of Traditional Chinese Medicine
Course: Surgery of Chinese Medicine
Keywords: Propionibacteria Erythromycin Clindamycin Tetracycline 23SrRNA ermX ermX (cj) Tn5432
CLC: R758.733
Type: PhD thesis
Year: 2014
Downloads: 2
Quote: 0
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Abstract


Abjective To evaluate the susceptibility test of propionibacteria and determine whether erythromycin resistant propionibacteria is due to the mutation in23S rRNA and Tn5432with ermX located plus two copies of IS1249flanked was transferred to propionibacteria. To determine the susceptibility test of propionibacteria to berberine.Methods Samples were collected from acne lesions of84acne patients. All samples were cultured in anaerobic conditions and identified by o sequencing of16s rRNA and23s rRNA respectively. The susceptibility test of propionibacteria isolated from acne patients to erythromycin and clindamycin was tested with E-test23S rRNA, ermX, ermX (cj), IS1249a and IS1249b was amplified and sequenced respectively. To determine the susceptibility test of propionibacteria to berberine with broth microdilution method. Real time PCR was evaluated to detect if erm was regulated by berberine.Results72Propionibacterium acnes and12Propionibacterium avidum were isolated respectively. Isolates resistant to erythromycin (29/84,34.52%) were observed and all isolates were susceptible to tetracycline. Isolates resistant to erythromycin were observed in P.acnes (26.39%,19/72) and P.avidum (83.33%,10/12) respectively.19P.acnes isolates and10Ravidum isolates were all high resistant to erythromycin.16P.acnes isolates were high resistant (MIC>256μg/ml) and3susceptible to clindaymcin respectively.10P.avidum isolates were all high resistant to erythromycin and clindaymcin (MIC>25μg/ml). In7P.acnes isolates, cross high resistant to erythromycin and clindamycin was associated with an A→G transition at a position cognate with Escherichia coli23S rRNA2058. These isolates were resistant to>256μg/ml of erythromycin and clindamycin respectively. In4P.anees isolates, high resistant to clindamycin and partly susceptible to clindamycin was associated with an A→G transition at a position cognate with E.coli23S rRNA2059. ermX gene located in Tn5432with two copies of IS1249was detected in the other8P.acnes isolates and2Ravidum isolates. These isolates were all high resistant to clindamycin and erythromycin. ermX(cj) gene was detected in the other8P.avidum isolates which shows99%homology with Corynebacterium jeikeium ermX (cj), while94%with P. acnes ermX. Berberine inhibited propionibacteria(MIC1-8μg/ml). The mutation of23S rRNA and erm gene weren’t regulated by berberine.Conclusion Antimicrobial resistance in propionibacteria in this population has a higher prevalence than those reported in Japan and Korea and follows a similar pattern to findings in Europe. No isolates were resistant to tetracycline. Clinical resistant propionibacteria to erythromycin is associated with an A→G transition at a position cognate with E.coli23S rRNA2058and2059respectively, erm located in Tn5432and ermX(cj) also account for the resistance. Berberine inhibited P.acnes, which provides a good choice for treatment of acne.

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CLC: > Medicine, health > Dermatology and Venereology > Dermatology > Skin appendages and organs diseases > Sebaceous gland diseases > Of acne vulgaris ( acne)
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