Dissertation > Excellent graduate degree dissertation topics show

DDP and Hyperthermia for Intraoperative Autotransfusion in Liver Cancer Surgery: Elimination of Carcinoma Cells and Effect to Erythrocytes

Author: ZhouJinFeng
Tutor: LeiWeiFu；YuJinGui
School: Shandong University
Course: Anesthesiology
Keywords: tumer cells erythrocytes DDP hyperthermia intraoperative autotransfusion
CLC: R735.7
Type: Master's thesis
Year: 2011
Downloads: 18
Quote: 0
Read: Download Dissertation

Abstract


Objective To investigate the role of DDP and hyperthermia for intraoperative autotransfusion to eliminate carcinoma cells and the effect to erythrocytes in liver cancer surgery.Methods Cultured hepatoma carcinoma cells were mixed with erythrocytes suspensions and separated to 6 groups by temperature and DDP concentration, A: 37℃and DDP 0μg/ml, B:37℃and DDP 100μg/ml, C:37℃and DDP 200μg/ml, D: 42℃and DDP 0μg/ml, E:42℃and DDP 100μg/ml, F:42℃and DDP 200μg/ml, then respectively treated for 30 min. After that, tumer cells were isolated from RBCs using density grandient centrifugation. Part of the isolated tumer cells were tested for inhibition ratio by MTT method. The rest tumer cells were cultured and their clone formations were checked. The isolated erythrocytes suspensions were tested for osmotic fragility and 2,3-DPG.Results The effect of eliminating carcinoma cells in a DDP centrifugation and temperature dependent manner (P<0.01). The inhibition ratio of tumer cells is more than 99% and there is no clone formation can be tested in group F. The osmotic fragility of RBCs is slightly modified in group F, however the hemolysis ratio is no more than 1% when the sodium chloride is 0.68%. There is no deference of the RBCs’ 2,3-DPG between before and after being treated in group A and group F (P<0.01)Conclusion When treated at 42℃and DDP 200μg/ml for 30 min, all hepatoma carcinoma cells in the recycling blood lost proliferation ability and there is no effect to the transport oxygen ability of the erythrocytes.

Related Dissertations

  1. The Effect of RNA Interference-mediated ERCC1 Gene on the Chemo-treatment Sensitivity of NSCLC in Vitro,R734.2
  2. Study of the Reversal Effect of Recombinant Human Endostatin on Chemotherapy Resistance in Human Lung Adenocarcinoma A549/DDP Cells,R734.2
  3. Anti-tumor Effect of Trastuzumab on Nude Mice Xenografts of Human Ovarian Cancer SKOV3 Cells and Its Synergistic Effect of Action with Docetaxel, Cisplatin,R737.31
  4. Application of Self-tuning Fuzzy Control Algorithm in the Temperature Control System for Tumor Intervention Hyperthermia,R197.39
  5. The Effect of 17-DMAG on Liver Cancer Cell Lines HepG2,R735.7
  6. Analysis of Clinical and Genetic for Malignant Hyperthermia,R441.3
  7. Study on the Expression and Signification of N-cadherin、bFGF、PCNA on the Neural Tube Defects Model in Golden Hamster Induced by Hyperthermia,R363
  8. Development and Application of Tumor-targeting Magetic Nanoparticles for Hyperthemia,TB383.1
  9. Correlation Study of DNA-PKCs and Osteosarcoma Chemotherapy Resistance,R738.1
  10. An Experimental Study of Radiation Therapy Combined with Hyperihermia on Angiogenesis of Hepatocellular Carcinoma in Mice,R735.7
  11. The Cytotoxic Effect and Mechanisms for Thermochemotherapy of 5-FU to Human Gastric Cell Line,R735.2
  12. Inhibition of CD26/DDPⅥ Attenuates Ischemia/Reperfusion Injury During the Early Post-lung Transplantation,R655.3
  13. Relationship between NF-κB Expression and Cisplatin Resistance in Lung Adenocarcinoma Cells and Mechanism of Evodiamine in Reversion of Multidrug Resistance,R734.2
  14. Study on High Performance Whole Body Hyperthermia Via Combining Blood Vessel Heat Transport and Microwave Irradiation,R730.5
  15. The Effect of Standard of Pure Puerarin and Pueraria Crude Extract on Cisplatin Inhibit Growth of Lung Cancer Cell H446,R734.2
  16. The Effect of Cisplatin Combined with Hyperthermia in Different Temperature on the Cell Growth and Metastasis of Human Ovarian Cancer Cell Line Skov-3,R737.31
  17. Based on S3C9454 Induction Nano Ferrite Heating Experimental Cancer Treatment Instrument Control System Design,R318.6
  18. Effect of the Cancericidal Solutions Commonly Used in Operating Room and Thermotheraphy on Lung Cancer Cell Line A549,R734.2
  19. The Relationships between PTEN Expression and Cisplatin Resistance in Ovarian Cancer Cells,R737.31
  20. Stable Reversal of Multidrug Resisitance in A549/DDP Cells by RNAi Targeting MRP1 Gene,R734.2
  21. Research on Measurement the Resistivity-temperature Properties of Liver Tissue and Monitoring of Electrical Impedance Tomography,R730.5

CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Liver tumors
© 2012 www.DissertationTopic.Net  Mobile