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Objective:Observe the effect of exercise joint of A. camphorata to the blood-lipid changes, vascular endothelial injury, and oxidized low-density lipoprotein (ox-LDL) in hight-fat diet rats, to explore the mechanisms of exercise joint of A.camphorata effect on high-fat diet rats’ aortic endothelial morphology damageintervention, and provide a theoretical basis for further evidence of the exercise joint of A.camphorata cholesterol fed rat aortic injury prevention mechanism for theapplication.Methods:The40male SD rats were randomly divided into control group (C), high fat diet group ((HF group), swimming+high-fat diet group (HE), A. camphorata+high-fat diet group (HI), camphorate+swimming+high-fat diet group (HIE). HE group and HIE group90min swim with non-weight-bearingsix days a week for12weeks. Measure and record the changes of rats’ body weight once a week during the experment. Measure the serum TC, TG, LDL HDL, MDA, ox-LDL sICAM-1content, aortic protein concentration, and the LOX-1of arota, P38MAPK, NF-κB content.Results:1、Compared with control group, the body weight of the high fat diet group increased significantly (P<0.01); serum TG, TC, LDL-C levels were significantly increased (P<0.01), HDL-C level was significantly decreased (P<0.01); MDA, ox-LDL and sICAM-1levels in serum were significantly increased (P<0.01); LOX-1, P38MAPK, NF-κB of aorta were significantly increased (P<0.01); morphological observation, endothelial damage was significant. Note the high-fat diet in rats weight gain, elevated blood lipids, and changes in related factors and endothelial damage modeling success of this experiment.2、Scanning electron microscopy, the endothelial cells of control rats was flat strip, regularly arranged smooth surface, longitudinal folds clearly visible cell boundaries, with a certain direction, consistent with the direction of blood flow. Rat endothelial cells compared with the control group, the abnormal high-fat diet group rat endothelial cell shape and arrangement, longitudinal wrinkles is not clear, cells become sparse fuzzy, surface uplift, insect bite-like structure, showing lipid deposition on the surface, accompanied by cauliflower-like plaque; instructions, high-fat diet can lead to rat aortic endothelial cell damage. High-fat diet+exercise group cell morphology, are able to see, but the cell gap and the lipid attached to its surface, the extent of the damage a high fat diet group; description of swimming exercise on rat aortic endothelial cells injury have a preventive effect. The high-fat diet+A. camphorata rats high fat rats endothelial cell morphological differences are not obvious, the surface is still a lot of lipids and insect bite-like holes; A. camphorata on rat aortic endothelial cell damage although the change but did not change significantly. A. camphorata+swimming+high fat diet group and normal group was similar in morphology, endothelial surface changes significantly larger cell gap and the surface of a small amount of lipid. Explain the effect of A. camphorata+swimming rat aortic endothelial cell injury prevention.3、Compared to group HF, the body weight of HE group was significantly lower (P<0.01); serum TG, TC and LDL-C level significantly reduced (P<0.01), HDL-C levels were significantly elevated (P<0.01); of MDA, ox-LDL and soluble intercellular adhesion molecule-1levels in serum were significantly lower (P<0.01); aorta of LOX-1, P38MAPK, NF-kB was significantly decreased (P<0.01); high fat diet groupA. camphorata+high-fat diet group rats body weight decreased significantly (P<0.05); serum TG reduce very significantly with the (P<0.01), the TC, LDL-C, have decreased but not significant with (P>0.05), the HDL-C also tended to increase but not significantly (P>0.05); serum MDA levels of sICAM-1levels were significantly lower (P<0.01), ox-LDL levels decreased but not significantly (P>0.05); aorta of LOX-1was significantly decreased (P<0.05), of P38MAPK of NF-κB content was significantly lower (P<0.01); compared with the high fat diet group, the A. camphorata+swimming+high fat diet group weight loss is very significant (P<0.01);the TC,TG, LDL-C in serum to reduce very significantly with the (P<0.01), HDL-C, increased very significantly with the (P<0.01); serum of MDA by ox-LDL levels of sICAM-1content reduce the very significant with (P<0.01); aorta of LOX-1,P38MAPK, NF-kappa B decreased significantly (P<0.01); description of exercise on weight control, lipid changes and their associated factor levels to a certain extent.4、Swimming+high fat diet group, the A. camphorata+swimming+high-fat diet group, body weight of rats decreased but not significantly (P>0.05); serum of TG, TC, LDL-C tended to decrease but not significantly(P>0.05), HDL-C increased trend was not significant (P>0.05); MDA,ox-LDL,sICAM-lin serumlevels by tended to decrease but not significantly (P>0.05); aortic LOX-1,P38MAPK,NF-κB content reduce the trend but not significant (P>0.05); description of Antrodia camphorata+swimming group than a simple swimming on body weight control, changes in blood lipids and by ox-LDL, LOX-1, P38MAPK, NF-kappa B, sICAM-1level changes in the role of difference.5、A. camphorata+high fat diet group, the A. camphorata+swimming+high-fat diet group, body weight of rats decreased significantly (P<0.01);TG, TC, LDL-C serum levels were significantly lower (P<0.01), the HDL-C tended to increase but not obvious (P>0.05); serum MDA content significantly with lower (P<0.05), ox-LDL content is very significant with (P <0.01), levels of sICAM-1content have lower trend but not significant(P>0.05); LOX-1, P38MAPK, NF-κB in the aorta decreased significantly (P<0.05); description of A. camphorata+swimming group than in the pure Antrodia camphorata group on body weight control, blood lipid changes and associated factorsthe level of change effect is better.Conclusion:1、High-fat diet can cause the increasation of rats’ body weight, TC, TG,LDL,HDL and obvious injury of aortic endothelial cells.2、Exercise can prevent the increasation of high-fat diet rats’ weight, blood TC, TG, LDL, HDL and the cellinjury of aortic endothelial; A. camphorata can prevent high-fat diet in rats body weight, blood lipids TC, TG,LDL HDL laortic endothelial injury, while the role of the effect is not obvious; A. camphorata+exercise can prevent high-fat diet rats weight, blood lipids in TC, TG and LDL and the HDL increased aortic endothelial damage, the effect of the best from areflection of the exercise by some mechanism to accelerate the utilization of A. camphorata, is the feasibility of drug and non-drug combination therapy of related diseases caused by the fat. Prompt treatment of related diseases caused by high-fat environment should be taken to enhance the efficacy of multi-factor synergy.3、Exercise of A. camphorata to prevent high-fat diet in rat aortic injury possible mechanisms are: the role of reducing the growth of high-fat diet on body weight, lower blood lipids and lipid peroxidation, inhibition of the formation of ox-LDL, reducing its specificityLOX-1expression of the receptor, reducing the toxicity of fat ox-LDL in endothelial cells, to mitigation P38MAPK/NF-κB activation in endothelial, reducing ICAM-1expression, reduce lipid deposition, which prevent high-fat diet-induced aorticdamage.
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