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Fabrication and Application of Photoelectrochemical Sensors Based on Quantum Dot and Enzyme Nanocomposites

Author: ZhengMin
Tutor: LiuShaoQin
School: Harbin Institute of Technology
Course: Biomedical Engineering
Keywords: Layer-by-layer assembly Quantum dots Glucose oxidase Acetylcholinesterase
CLC: TP212.3
Type: Master's thesis
Year: 2011
Downloads: 63
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Abstract


Photoelectric biosensor is based on the photoelectric effect , the light signal is converted to electrical signal . The advantage of this sensor as the sensing signal due to the use of the light generated current , only one light source and a current meter , the method is simple and reliable, and can avoid the drawbacks of the common sensor . Therefore , in this paper , we prepared the different structure of the enzyme biosensors , such as glucose biosensors and acetylcholinesterase optoelectronic biosensors , glucose and organophosphorus pesticide residues were detected photocurrent and the relationship of the concentration of the analyte . We use layers of self-assembly method in the mesoporous TiO 2 film surface alternating deposition of CdSe _AT_ CdS QDs and PEI -[ cobalt ( o -phen ) 3 < / sub > ] 2/3 prepared TiO 2 / CdSe @ CdS nanocomposites films . As the electron mediator [cobalt (o-phen) 3] 2/3 added , the film may be higher, and a stable light current . Secondly, we have used as a model to the glucose oxidase (GOD) , and use of TiO 2 / CdSe @ CdS / GOD nanocomposite film to achieve the detection of glucose . When the system was added glucose , glucose by the glucose oxidase catalyzed oxidation , electrons generated can be passed directly to the quantum dots or Cobalt ( o -phen ) 3 ] 2/3 < / SUP > and cause the light generated the current increases with the increase of the concentration of glucose . The sensor showed a good reproducibility and storage stability . Finally, we will acetylcholinesterase (AChE) deposited on TiO 2 / CdSe @ CdS multilayer film surface , build the TiO 2 / of CdSe _AT_ CdS / AChE structure nanocomposite films . The ultraviolet and visible light of CdSe _AT_ CdS nanoparticles by light excitation generated holes and electrons, acetylcholinesterase hydrolyzate - thio choline to physical as an electron hole oxidation , photoexcited electrons generated is transmitted to the electrode and generate photocurrent. When the enzyme electrode is in contact with parathion , parathion with acetylcholinesterase binding thereby inhibiting the enzyme activity , reduced thio choline , resulting photocurrent decreases . According to this principle , we get different concentrations of parathion photocurrent rejection rate of the standard curve , can be tested on the actual samples to the standard curve .

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CLC: > Industrial Technology > Automation technology,computer technology > Automation technology and equipment > Automation components,parts > Transmitter ( converter),the sensor > Biological sensors,medical sensors
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