Dissertation > Excellent graduate degree dissertation topics show

Possible Mechanism of miR-24Inhibits Hypothermia-induced Apoptosis of Cardiaomyocytes

Author: YangJiao
Tutor: ChengXiaoShu
School: Nanchang University Medical College
Course: Internal Medicine
Keywords: Cardiaomyocyte hypothermia nduce apoptosis miR-24 Bim caspase-3
CLC: R329.2
Type: Master's thesis
Year: 2013
Downloads: 30
Quote: 0
Read: Download Dissertation


Objective:To investigate whether the hypothermia environment (25°C) can induce theapoptosis of cardiaomyocytes, and to identify whether miR-24can inhibithypothermia-induced apoptosis of cardiaomyocytes and the possible mechanism.Methods:H9C2cardiaomyocytes were used for all experiments.(1)H9C2cardiaomyocyteswere respectively treated for4h、8h、16h、24h、32h、40h and48hours at25°C inthe low temperature incubator, and normal group as control, then flow cytometricassay was used for measuring the effect of apoptosis, western blot was used to detectthe level of caspase-3and Bim protein, and Quantitative real-time polymerase chainreaction(qRT-PCR) was used to detect the expression level of miR-24. According tothe apoptosis rate we choose24hours as the hypothermia stress time in the nexttransfection experiment.(2)Using Lipofectarmine-2000to mediate transfection, theartificial synthetic miR-24mimic and miR-24inhibitor were transfected tocardiaomyocytes, then fluorescence microscope was used to observe whethertransfection is successful and the qRT-PCR detect the expression level of miR-24and estimate the transfection efficiency.(3)After the transfected cardiaomyocyteswere stressed by low temperature for24hours, we also use flow cytometry to detectthe apoptosis rate, western blot to detect the expression of caspase-3and Bim proteinand the qRT-PCR to detect the expression level of miR-24.Results:(1)After the cardiaomyocytes were stressed by low temperature respectivelyfor4h、8h、16h、24h、32h、40h and48hours, compared with normal control group,the apoptosis rate was gradually increasing and reached a peak at24hours (P<0.05);the expression of apoptosis market protein caspase-3was also gradually increasingand reached a peak at24hours(P<0.005); the expression level of miR-24wasgradually decreasing and reached a slack at24hours(P<0.01); the expression level of Bimprotein was also gradually increasing after16hours (when the miR-24level droppedsignificantly) and reached a peak at24hours(P<0.005).(2) Transfection concentration for100nm, the level of miR-24was highlyexpressed for8times by miR-24mimic and was low expressed for79%by miR-24inhibitor (P<0.005).(3) Compared with the hypothermia control group, in the miR-24mimic groupafter hypothermia stress the apoptosis rate was significangtly decreased(P<0.01),and also the expression level of caspase-3(P<0.05) and Bim protein(P<0.05); Onthe contrary in the miR-24inhibitor group after hypothermia stress the apoptosisrate was significangtly increased, and also the expression level of caspase-3and Bimprotein.Conclusion:The25C low temperature can induce cardiaomyocyte apoptosis, the miR-24caninhibit the hypothermia-induced apoptosis in cardiaomyocytes, and its mechanismmay be associated with inhibiting the expression of promote apoptosis protein Bim.

Related Dissertations

  1. The Study on Cryopreservation and Mechanism of Freezing Injury on the Spermatozoa of Coelomactra Antiquate,S968.3
  2. The Anti-tumor Effect of CADPE and Inducing Apoptosis in Human Gastric Cancer Cells,R735.2
  3. TRAIL in the regulation of tumor invasion CD4 ~ CD25 ~ Treg,R730.2
  4. The Study of Lead Exposure on Cyprinus Carpio Ovary Epithelial Cells,X174
  5. Effects of Diclazuril on G3PDH in Second-generation Merozoites of Eimeria Tenella,S858.31
  6. The Effects and Preliminary Study of PCV2 on Ca2+ Signal in Lymphocytes of Piglets,S858.28
  7. Expression of β-Catenin in Pig’s Ovary and the Effect of β-Catenin on Porcine Granulosa Cells Apotosis and Steroidogenesis Related Enzyme,S828
  8. Induced Combination Apoptosis by FB1 and AFB1 in Vero Cell,S856.9
  9. Effects of BMPR-IB Gene Silencing by Small Interfering RNA on Apoptosis of Porcine Pollicular Granulosa Cells and Ecpression of BMP Pathy-Way-Ralted Genes,S828
  10. Study of Melittin on Tumor Inhibition and Part of Mechanism of Human Hepatocellular Carcinoma HepG-2 Xenograft in Nude Mice,R735.7
  11. The Expression and Mechanism of NF-κ B and IL-6 after 90% Portal Vein Ligation in Rats,R657.3
  12. The Impact of Histone Deacetylase Inhibitor Trichostatin a on Proliferation and Apoptosis of Porcine Granulosa Cells,S828
  13. Initial Research on the Expression and the Significance of Cx-43、 Caspase-3 and MMP-2 in Endothelial Cells in Atherosclerosis in Human Coronary Artery,R541.4
  14. Inhibition of Conventional Chemotherapy Combined with Metronomic Chemotherapy on Breast Cancer Xenografts in Nude Mice,R737.9
  15. Inhibitory Role of Plasmodium Yoelii Circumsporozoite Protein on the Proliferation and Survial of Tumor Cell,R730.5
  16. Expression and Clinical Significance of PTEN and Caspase-7 in Gastric Cancer and Precancerous Lesion,R735.2
  17. The Protective Effects and Action Mechanism of Trans-resveratrol on Rats Hippocampus Injury by Aβ25-35,R277.7
  18. Effect of Bufalin on Proliferation and Induce Apoptosis of Vascular Endothelial Cells EAhy926,R285
  19. Effects of Dachengqi Decoction on the Expression Levels of Bcl-2 and Bax Gene of Smooth Muscle Cells of Small Intestine in Rat with Multiple Organ Dysfunction Syndrome,R285.5
  20. Anti-cancer Effects of Tanshinone ⅡA Microemulsion for H22 Murine Hepatoma Cells Both in Vitro and in Vivo,R285.5
  21. Effects on Recombinant Human Interleukin-10 of IL-2, IFN-γ, GCs, T Cell Apoptosis in Transplantation of the Rabbit Skin,R392

CLC: > Medicine, health > Basic Medical > Human morphology > Human histology > Human cytology
© 2012 www.DissertationTopic.Net  Mobile