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Construction of Novel Dopamine Sensors Based on Covalent Modification Procedure

Author: WuYanYing
Tutor: HeXingQuan
School: Changchun University of Science and Technology
Course: Applied Chemistry
Keywords: dopamine sensor electroabsorption methoxypolyethylene glycol β-cyclodextrin-poly(N-isopropylacrylamide) 4arm-PEG-NH2 iron-tetrakishydroxyphenylporphyrin modified electrode
CLC: O657.1
Type: Master's thesis
Year: 2014
Downloads: 4
Quote: 0
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Abstract


Dopamine (DA) is an important neurotransmitter molecule of catecholamine and widely present in the peripheral nervous system. An abnormal amount of DA may lead to serious diseases. So, researchers have put much effort into developing various methods for the determination of DA. Electrochemical methods have been successfully developed, which have significant advantages of simplicity, low cost and high sensitivity.We present a one-step covalent modification approach to fabricate three kinds of novel dopamine sensors through anchoring methoxypolyethylene glycol (MPEG) polymer, β-cyclodextrin-poly(N-isopropylacrylamide)(CD-PNIPAM) and four arm-polyethylene glycol-amine (4arm-PEG-NH2) polymer onto a glassy carbon electrode (GCE), respectively. The dopamine sensors exhibited a good electrocatalytic activity towards the oxidation of dopamine compared with bare GCE. We investigated the effect of pH and scan rate on the CV response of DA at the modified GCEs in this study. The lowest detection limits for DA were estimated as4.68×10-8M,3.34x10-8and4.68×10-8M by differential pulse voltammetry (DPV) method, respectively. The DA sensor exhibits good sensitivity, selectivity and stability, and has been applied for the determination of DA in real samples.We also develop a two-step covalent modification approach to fabricate a novel dopamine sensor through anchoring iron-tetrakishydroxyphenylporphyrin (Fe(OH)4P) and4arm-PEG-NH2onto a glassy carbon electrode (GCE). The electrode presented an excellent electrocatalytic activity towards the oxidation of DA by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) methods, the modified electrode was used for the determination of DA at a linear range of0.2-125μmol L-1with a detection limit of1.02×10-8M. We also investigated the effect of pH and scan rate on the CV response of DA at the (Fe(OH)4P)/4arm-PEG-NH2modified GCE. The sensor possesses high selectivity, sensitivity, stability and a low detection limit, which makes it suitable for the determination in serum samples.

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Electrochemical analysis
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