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Synthesis of pH-sensitive Fluorescent Probes and Their Applications in Monitoring pH in Living Cells

Author: CuiPeng
Tutor: GaoFeng
School: Anhui Normal University
Course: Analytical Chemistry
Keywords: pH-sensitive probes Rhodamine derivate silicananoparticales cell imaging pH monitoring
CLC: O657.3
Type: Master's thesis
Year: 2013
Downloads: 50
Quote: 0
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Abstract


Among analytical methods, fluoremetry has some distinguishedadvantages, such as high sensitivity, various characteristic parameters,simple operation and other merits to promote the fluoremetry widely usedin chemo/biosensing. However, most of fluorophore have somedrawbacks such as photobleaching, low quantum yield, and toxic to vivosamples, which prevent the wider application of fluoremetry. Accordingdetected species, Synthetic fluorophore is a powerful tool in cell biologyfor the nondestructive measurement of intracellular species owing totheir simplicity and sensitivity. The alert way is dope the fluorophore inan carrier, the fluorophore doped carrier has following characteristics,excellent cell membrane, high quantum yield，good photostability andbiocompatibility，uniform and tunable size. And the properties can notchange compared with free dyes. The silica nanoparticals are in goodagreement with above demands.In this paper, A water solubility rhodamine derivative, twofluorescent dyes doped silica nanoparticalles were prepared and appliedto monitor pH in living cells. The details are as follows:1. A rhodamine-derivated and water-soluble fluorescent probe for intraand extra-cellular pH sensing and imaging. 2. Synthesis of dual-emission core-shell fluorescent silica nanoparticalesvia layer by layer and their applications in monitoring pH in living cells

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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