Dissertation > Excellent graduate degree dissertation topics show

Study on Removing Effect of Phospholipids in Crude Soybean Oil by Phospholipase C

Author: YangJiao
Tutor: JinQingZhe
School: Jiangnan University
Course: Cereals, Oils and Vegetable Protein Engineering
Keywords: phospholipase C crude soybean oil phospholipids-removing optimization fac-tors
CLC: TS225.13
Type: Master's thesis
Year: 2013
Downloads: 53
Quote: 0
Read: Download Dissertation

Abstract


As a new method, removal of phospholipids by enzymes has many advantages. Com-pared to the tradition water and acid methods, enzymatic method uses little chemicals, waterand electricity consumption, simple operation and little loss of oil. Phospholipids can be hy-drolyzed by phospholipase C (PLC) to diacylglycerols (DAG) and phosphate esters. Com-pared to phospholipids-removing by phospholipase A(PLA), phospholipids-removing by PLCcan be completed fast. More little loss of oil and increase of DAG content can be gotten afterPLC reaction. Therefore, phospholipids-removing by PLC is a promising method. Processoptimization and factors of PLC reaction have been studied in this thesis.First, the selectivity of PLC was studied. After analyzing the PLC reaction results ofthree kinds of crude soybean oil with high, middle and low phosphorus content, conclusionwas drawn that PLC reaction was fast.85.0%of phosphorus was removed in0.5h and theremoval of hydratable phospholipids (HP) was much higher than nonhydratable phospholipids(NHP) during the first0.5h. High remove of HP in crude oil with high HP/NHP value whilelow remove of HP in oil with low HP/NHP value was gotten by PLC degumming. PLC reac-tion was suitable for crude soybean oil with low phosphorus content and HP/NHP.The process was optimized.The temperature was set at50℃, time1h, enzyme dosage30mg/kg, pH5.0, water content3%and stirring speed300r/min. The phosphorus contentwas21.2mg/kg in reacted oil while the DAG was0.32%. The results of orthogonal experi-ment were: enzyme dosage30mg/kg, temperature50℃, water content2%, stirring speed200r/min, pH5.0. The phosphorus content was17.0mg/kg in reacted oil while the DAG was0.33%.Three kind factors were studied to reduce the phosphorus deeply. The predominant effectof45%(w/w) citric acid pretreatment before PLC reaction was to adjust pH. Compared to theresults of reacted EDTA chelated oil by PLC, Mg2+,Ca2+,Fe3+,Zn2+,Cu2+all had suppressingeffects on PLC reaction. The suppressing effect increased with the increase of ion concentra-tion, especially Fe3+. When PLC and PLA were used together to phospholipids-removing, thephosphorus content reduced under5mg/kg after0.5h, no matter the reaction condition andratio of PLC/PLA. The enhancement resulted from the increasing of contact area. No obviousenhancement was observed when non-ion surfactant Span20, Span40, Span80, Tween20andTween80were added, neither solvent-phase degumming by PLC.Finally, scale-up experiment demonstrated that there was no evident amplification effect,which layed a foundation for industrialization of PLC degumming.

Related Dissertations

  1. Development of the Platform for Compressor Optimization Design and Aerodynamic Optimization Design in the Transonic Compressor,TH45
  2. Investigation of Turbine S2 Stream Surface Direct Problem Aerodynamic Optimization Design,V235.11
  3. Reseach on Optimal Control of Elevator Group Based upon Ant Colony Algorithm,TU857
  4. Research on High Efficiency Interior Permanent Magnet Synchronous Motor,TM341
  5. Application of Interior-Point Theory for Reactive Power Optimization in Large Power Systems,TM714.3
  6. The Basic Research of Axial Flux Inductor Type High Temperature Superconducting Motor,TM37
  7. The Studies and Establishment of Ready-mixed Concrete Information-guided Price on the Basis of Risk Analysis,F407.92
  8. Studies on Fermentation Optimization, Purification and Enzyme Characteristics of Lipase from Aspergillus Oryzae FS-1,TQ925.6
  9. The True Estimations of China’s Unemployment and the Re-examination of Okun’s Law,F224
  10. Methane and Carbon Dioxide Fluxes from Spartina Alterniflora Marsh in Minjiang River Estuary,X502
  11. Optimization of Fermentation Conditions, Purification, Cloning and Expression of a Cold-active Lipase from Pseudomonas Sp.RT-1,TQ925
  12. Open pit production accidents studied because of the risk management measures,TD771
  13. Cellulose Pretreatment with Ionic Liquid for Enzymatic Hydrolysis,TQ352.1
  14. The Dynamic Performance Analysis of Oil Pumping Center and Structural Optimization of Improvement,TE933.1
  15. Finite Element Analysis and Optimization Design of a Tractor Cab,S219.02
  16. Effects of Climate Factors for Different Transplanting Dates on Quality and Ripe Tissue in Flue-cured Tobacco,S572
  17. Methane Emission from Typical Plants in Mid-subtropical of China: Influencing Factors and Source of Preliminary Discussion,S718.4
  18. Drug Diffusion in Optimizing Control and Its Numerical Method,R91
  19. Environmental Lead Exposure Among Preschool Children in Hefei, China: Blood Lead Level and Risk Factors,R179
  20. Strain Screening of Hainanmycin Production and Fermentation Process Optimization,TQ927
  21. Establishment of the Rat Bone Mesenchymal Stem Cell Lines Stably Expressing ChM-I,R329

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Edible oils and fats processing industry > A variety of edible oil > Vegetable oil > Soybean oil
© 2012 www.DissertationTopic.Net  Mobile