Dissertation > Excellent graduate degree dissertation topics show

Development of a Biomimetic Enzyme-Linked Immunosorbent Assay Method of Molecularly Imprinted Film for Ractopamine

Author: LuJinPing
Tutor: FangGuoZhen
School: Tianjin University of Science and Technology
Course: Of Food Science
Keywords: RAC Organic-inorganic hybrid technology Molecular imprinted film Biomimetic enzyme-linked immunosorbent assay
CLC: TS251.7
Type: Master's thesis
Year: 2011
Downloads: 41
Quote: 0
Read: Download Dissertation

Abstract


In this thesis, a highly selective imprinted membrane was synthesized by an organic-inorganic hybrid technology using ractopamine (RAC) as template, methacrylic acid as functional monomer, γ-(methacryloyloxyethyl) trimethoxysilane as cross-linker. The polymerization conditions were discussed in details. The imprinted membrane was evaluated by FT-IR and static and kinetic adsorption experiments. A sensitive direct competitive biomimetic enzyme-linked immunosorbent assay (BELISA) was developed with the molecularly imprinted film as artificial antibody. And the imprinted film exhibited an antibody-like binding ability, rapid adsorption speed, high stability and hydrophilic nature, which was particularly advantageous and suitable for the BELISA development.The results showed that the molecularly imprinted film exhibit higher adsorption capacity and selective ability than that of non-imprinted film. The imprinted film had fast uptake kinetics,0.46μg well-1of binding capacity was obtained within a short shaking period of10min at RAC concentration of20mg L-1, which was67.5%of the saturated binding capacity, and the adsorption equilibrium was almost reached within60min. The established ELISA method gave a good sensitivity (IC50:15.77μg L-1) and a low detection limit (IC15:0.01μg L-1) for RAC under the optimum condition and it was applied to the determination of RAC in urine and pork samples with recoveries ranging from77.7%to108.9%(urine) and from93.5%to101.1%(pork). The obtained results, which correlated well with those gained from the traditional HPLC method (R2=0.9974(urine),0.9922(pork)), demonstrated that the developed ELISA method was reliable for RAC determination in real samples.

Related Dissertations

  1. Synthesis and Characterization of Neutral Phenoxyamido Lanthanide Complexes and Their Catalytic Behavior,O627.3
  2. Synthesis, Characterization and Catalytic Activity of Bridged Phenolate (Alkoxylate) Lanthanide Complexes,O627.3
  3. Preliminary Study of TCE Removal by GAC/ZVI and GAC/ZVI/Pd,X52
  4. Postal financial network the RAC optimization technology Research and Implementation,TP311.13
  5. Effect of the Expression of StAR mRNA after Treatment of Ractopamine in Testis of Adult Rat,S859.8
  6. Research and Design on the Integration of Production, Broadcast, and Deposit of Digital TV Platform,TN948.4
  7. Study on Recycling and Reuse of Waste Concrete,TU528
  8. Rac- metolachlor and its S- enantiomer of the chiral ecological differences in toxicity studies,X173
  9. Fire Insurance Premium Rate Floating Research of Storage Firms Based on Fire Assessment,X932
  10. Clenbuterol , ractopamine residues of veterinary drugs immunochromatographic detection method,S859.84
  11. Development of an Colloidal Gold Immunochromatographic Strip for Detection of Ractopamine Based on Monoclonal Antibody,S859.84
  12. The Research and Simulation of Radio Admission Control in WCDMA System,TN929.533
  13. The two typical chiral pesticides ecotoxicological Differences,X592
  14. Panzhihua Iron and financial management of information technology research and application practice,TP315
  15. Expression Vector Construction and Functional Analysis of Cotton Rac Protein Gene (GhRacB),S562
  16. Functional Analysis of Cotton (Gossypium Hirsutum L.) Rac Protein Gene (GhRacA),Q943
  17. A Comparative Study of Rac-and S-metolachlor Toxicity to Earthworm,X174
  18. Research on the Different Effects of Metolachlor and Its S-enantiomer on Plant Cytochrome P450 and Soil Enzymes,S482.4
  19. The Toxic Mechanisms of Metolachlor to Scenedesmus Obliquus,X173
  20. Synthesis of Lanthanide Amides Supported by Phenoxy(quinolinyl)amide Ligand and Their Catalytic Behavior for Polymerization,O641.4
  21. Study on the Polymerization of Rac-Lactide and the Catalysts,TQ316.3

CLC: > Industrial Technology > Light industry,handicrafts > Food Industry > Slaughtering and meat processing industries > Product standards and testing
© 2012 www.DissertationTopic.Net  Mobile