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The purpose of behavioral and electrophysiological evaluation method, to investigate the sensitivity of neonatal fentanyl analgesia on visceral pain sensitive pups visceral pain; observe the pups lumbosacral spinal cord dorsal horn Fos protein expression to explore neonatal fentanyl Nigeria analgesic mechanism of inhibition of pups chronic visceral pain sensitivity, abdominal pain-related functional gastrointestinal disorders diagnosis, treatment and prevention provide a theoretical basis for children. Method (1) 32 SD neonatal rat pups visceral pain-sensitive model by a factorial design divided into four groups (n = 8), A1B1 group: neonatal repeatedly colorectal irritation (CI), the establishment of neonatal CI intraperitoneal injection of fentanyl Nepal; A1B2 Group: Neonatal repeated CI, create a highly sensitive pups visceral pain model, neonatal CI intraperitoneal injection of saline; the A2B1 group: neonatal did not receive CI, neonatal intraperitoneal injection of fentanyl; the A2B2 group: neonatal did not receive CI neonatal intraperitoneal injection of saline. Model group suckling mice born 8d start giving CI, 1 day, 7 consecutive days, the control group was not given to the CI, conventionally reared pups of (6wk), observed in rats at different pressures colorectal distension abdominal wall withdrawal reflex score, visceral pain threshold and external oblique discharge measurements for visceral pain sensitivity evaluation. (2) 32 only neonatal SD rats as the packet processing. The conventionally reared pups of (6wk age), L6-S2 spinal cord specimens spinal cord Fos-positive cell count and integral optical density semi-quantitative analysis, the application of immunohistochemistry and computer image analysis system. Using SPSS13.0 software package for statistical analysis, alpha = 0.05 significance test standards. Results ① increases with CRD pups AWR scores; AWR score by modeling and neonatal CI preventive intraperitoneal injection of fentanyl impact modeling AWR scores can rise in CRD20 mmHg, 40mmHg, 60mmHg significantly different (P lt; 0.001) in CRD80mmHg, no significant difference (P gt; 0.05) Neonatal CI preventive intraperitoneal injection of fentanyl can make the AWR scores lower in CRD20mmHg significantly different (P lt; 0.05), CRD pressure to 40mmHg 60mmHg 80mmHg when no significant difference (P gt; 0.05). Both in CRD20mmHg, 40 mmHg, 60 mmHg when interaction (P lt; 0.05), there was no interaction (P gt; 0.05) at 80mmHg. Modeling so that the pups significantly reduce the pain threshold, neonatal CI preventive intraperitoneal injection of fentanyl pups pain threshold was significantly higher, the difference was statistically significant (F = 4.421, P lt; 0.05; F = 108.08, P lt; 0.001), both interaction (F = 33.43, P lt; 0.001). ② increase with the CRD, pups external oblique discharge increased volatility; the pups external oblique discharge amplitude modeling and neonatal CI preventive intraperitoneal injection of fentanyl impact, modeling allows the external oblique discharge The amplitude increases in CRD15mmHg, 30mmHg, 45mmHg significant difference (P lt; 0.05), when no significant difference (p GT; 0.05) CRD60mmHg, 75mmHg. Neonatal CI preventive intraperitoneal injection of fentanyl external oblique discharge amplitude can reduce the CRD pressure of 15mmHg and 30mmHg at 45 mm Hg significantly different (P lt; 0.05). CRD pressure as 60mmHg and 75mmHg was no significant difference (P gt; 0.05). The both in CRD30mmHg interaction (P lt; 0.05), CRD15mmHg 45mmHg, 60mmHg, 75mmHg when there was no interaction (P GT; 0.05) ③ modeling allows the the pups lumbosacral spinal cord dorsal horn FLI cell number and FLI cells integrated optical density value was significantly higher neonatal CI prophylactic intraperitoneal injection of fentanyl, the pups can lumbosacral spinal dorsal horn FLI cell number and FLI cells integrated optical density was significantly lower; differences were statistically significant (F = 13.76, P lt; 0.05; F = 78.58, P lt; 0.001). The interaction between the two. Conclusion neonatal continuous CI can cause young rats pain threshold and the onset of chronic visceral hypersensitivity, and high sensitivity can continue to infancy pups visceral hypersensitivity model. Neonatal CI preventive intraperitoneal injection of fentanyl can lead to spinal cord neurons Fos protein expression decreased to prevent the pups spinal cord primary central sensitization, inhibition of pups chronic visceral pain highly sensitive formed.
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