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Lentiviral-mediated Targeted Angiopoietin-2 siRNA on Human Pancreatic Cancer PANC-Ⅰ Inhibition of in Vitro

Author: XuDeHua
Tutor: LiDeChun
School: Suzhou University
Course: General Surgery
Keywords: Pancreatic Cancer Ang2 RNA interference Small interfering RNA Lentiviral CAM Gene therapy
CLC: R735.9
Type: Master's thesis
Year: 2010
Downloads: 47
Quote: 0
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Abstract


Objective To observe the carrying lentiviral targeting angiopoietin- 2 siRNA on cultured human pancreatic cancer PANC-1 cells angiogenesis and invasion abilities role . LV-Ang2-siRNA transfection into human pancreatic cancer cell line PANC-1 , a green fluorescent protein (GFP) gene as a reporter gene , viral transfection efficiency observed using fluorescence microscopy and flow cytometry . By reverse transcription polymerase chain reaction to detect the the Ang2-mRNA and protein expression of PANC-1 cells , MTT method and flow cytometry cell cycle and apoptosis , tumor angiogenesis and metastasis invasion ability of the chick embryo and invasion assay . Results LV-Ang2-siRNA was successfully transfected into PANC-1 cells the FCM was detected by transfection efficiency of more than 95% . PANC-1 cells in the experimental group Ang2 mRNA and protein expression was significantly suppressed compared with the negative control group and blank control group . Transfer invasion ability of the cells of the experimental group decreased significantly reduced tumor angiogenesis significant difference was statistically significant ( P lt; 0.05 ) . Lower apoptosis rate of the three groups of cells , no statistically significant difference between the groups ( P gt ; 0.05 ) . Conclusion LV better transfection ability, PANC-1 cells may be mediated by the expression of the exogenous gene in PANC-1 . LV-Ang2-siRNA transfected PANC-1 cells to reduce PANC-1 the cell lines Ang2mRNA and the protein expression was significantly reduced tumor invasiveness and inhibit tumor angiogenesis , but its no significant role in the inhibition of proliferation and apoptosis .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Pancreatic tumors
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