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Objective To investigate the three-dimensional conformal radiation (3DCRT) mode and rapid radiation patterns on human hepatoma cell line BEL-7402 impact of changes in biological effects , surviving fraction and biological parameters changed circumstances ; observe human hepatoma cell line BEL-7402 and human hepatocytes QSG-7701 strain differences in radiosensitivity for clinical choose the right time of radiotherapy - dose fractionation model provides a theoretical basis . Methods 6MV X line two modes irradiated human hepatoma cell BEL-7402 ( including 0,1,2,3,5,7,9,10 Gy dose of 8 points , dose rate 3Gy/min): (1) rapid irradiation group , irradiation completion time 0-4min; (2) simulate three-dimensional conformal irradiation : irradiation completion time :13-15min. Into a cloning method to calculate survival fractions , mathematical model of multi-target click fitting curves , obtained SF2, D0, Dq other parameter values ??; 6MV X line single 5Gy irradiated cultured human hepatoma cell BEL-7402, human hepatocytes QSG -7701 , MTT assay , flow cytometry at different times after irradiation ( irradiation 6,12,24,36 h) cell viability , apoptosis , cell cycle . Results BEL-7402 human hepatoma cells rapidly simulate three-dimensional conformal radiation group and irradiated group D0, Dq, SF2 values ??were 1.78 and 1.69 ; 1.45 and 1.54 ; 0.625 and 0.654 ; QSG-7701 human liver cells 24 h after irradiation apoptosis rate highest ( 18% ) , while the BEL-7402 cells apoptosis 12h after irradiation highest rate ( 32% ) , the lowest survival rate ( 65% ) , two cells 12 h after irradiation apoptosis rate and survival largest difference (P lt; 0.01 ) . QSG-7701 after irradiation 24h, BEL-7402 after irradiation 12h, G2 / M phase was significantly increased ( respectively 23.03 ± 4.00% and 43.23 ± 6.44%). Conclusions 1 . Dimensional conformal radiation mode fractionated irradiation prolonged decline in biological effects ; 2 human hepatoma cell line BEL-7402 and human liver cell line QSG-7701 in the presence of radiosensitivity after irradiation differences ; 3 . Shorten each 3DCRT fractionated irradiation and fractionated irradiation time interval ( eg 12h) may improve clinical liver cancer radiotherapy .
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