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Experimental Study for Regulatory Effect of Growth and Advancing Chemosensitivity of Cisplatin on Human Gastric Cancer Cell Treated with Trichostatin A

Author: BiHuiFeng
Tutor: CaoWei
School: Suzhou University
Course: General Surgery
Keywords: Trichostatin A Gastric cancer Growing Cisplatin Sensitizing effect
CLC: R735.2
Type: Master's thesis
Year: 2010
Downloads: 26
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Abstract


Objective To observe the trichostatin A ( Trichostatin A TSA ) growth inhibition in vitro on human gastric cancer cell line SGC-7901 and cisplatin ( Cisplatin , DDP ) chemotherapy sensitizing effect , to explore its mechanism . ( 1 ) in vitro cultured human gastric cancer cell line SGC - 7901 . ( 2 ) MTT assay at different times with different concentrations of TSA on cell proliferation . (3 ) The experiment was divided into four groups of negative control , 400nmol / L , 800nmol / L , 1600nmol / L TSA , after 48h , flow cytometry and cell cycle distribution ; RT - PCR assay cells bcl-2 Caspase - 3 mRNA expression ; immunohistochemistry ( SP ) assay bcl-2, caspase-3 protein expression . ( 4 ) The experiment was divided into DDP alone , TSA DDP two groups , MTT assay two groups of inhibition of cell proliferation ; colony formation assay two sets of cell cloning efficiency ; Hochest 33258 staining the two sets of apoptosis . ( 1 ) MTT assay TSA can inhibit the growth of gastric cancer cell line SGC-7901 , has obvious time - and dose - dependent manner . ( 2 ) the TSA role of cells after 48 h , flow cytometry apoptosis significantly the G0/G1 of cells risen , S phase was decreased in a dose - dependent manner ( P lt ; 0.05 ) ; RT - PCR detection of cell bcl-2 mRNA expression decreased with the TSA concentration increased caspase -3 mRNA with the TSA concentration increased ( P lt ; 0.05 ) increased expression of bcl-2 protein ; immunohistochemical method (SP) shows cells with TSA concentration lower , caspase-3 protein with TSA concentration increased ( P lt ; 0.05 ) . ( 3 ) MTT assay 40 nmol / L TSA DDP group of gastric cancer cell growth inhibition rate compared with DDP group ( P lt ; 0.05 ) ; 40 nmol / L TSA the DDP group of cell colony formation rate compared with DDP group ( P lt ; 0.05 ) ; Hochest 33258 staining shows that 40 nmol / L TSA the DDP group of cell apoptosis compared with DDP group is more obvious . Conclusion TSA has significant growth inhibition of human gastric cancer cell line SGC-7901 , in a time- and dose-dependent manner ; TSA gastric cancer cells induced apoptosis and cell cycle arrest in G0/G1 phase ; TSA on gastric cancer cell proliferation inhibition may be related to reduced expression of bcl-2 and up-regulation of caspase-3 and induction of apoptosis ; certain concentration of TSA and DDP associated with , can enhance the sensitivity of gastric cancer cell line SGC-7901 DDP chemotherapy .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Gastric neoplasms
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