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The Effects of Selected Cyclooxygenase-2 Inhibition or Dual Cyclooxygenase-2/5-lipoxygenase Inhibition on the Proliferation of Esophageal Squamous Cell Carcinoma Cells

Author: WeiJingJing
Tutor: ZhuangZeHao
School: Fujian Medical
Course: Internal Medicine
Keywords: Cyclooxygenase- 2 5 lipoxygenase Esophageal squamous cell carcinoma Licofelone NS - 398 Cell Proliferation
CLC: R735.1
Type: Master's thesis
Year: 2010
Downloads: 22
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Abstract


Objective: To compare COX-2/5-LOX double chemical inhibition of enzyme activity and selective COX-2 enzyme activity chemical inhibition of cell proliferation of esophageal squamous cell carcinoma ( ESCC ) and its possible mechanism , looking for an effective way of esophageal cancer chemoprevention . : With COX-2/5-LOX double the pathway inhibitors licofelone and selective COX-2 inhibitor NS - 398 , the establishment of drug intervention group and blank control and solvent DMSO group . 25μM, 50μM, 75μM and 100μM concentration effect ESCC TE - 1 cells 24h and 48h , CCK - 8 ( tetrazolium monosodium salt ) assay cell proliferation ; RT - PCR and Western blot analysis before and after the intervention COX-2/5- LOX mRNA and protein expression ; ELISA assay downstream metabolites PGE2 and LTB4 content ; Flow cytometric cell cycle . Results : NS - 398 inhibition of cell proliferation was concentration-dependent , 50μM for 24h and 25 um , 50 microns and 100μM for 48h cell growth inhibition ( P lt ; 0.05 ) , the growth of other cells in each group with the control group no significant difference ( P gt ; 0.05 ) ; the cytostatic licofelone role in a dose- and time-dependent growth , in addition to 25 um processing 24h cells with the control group, no significant difference ( P GT ; 0.05 ) , higher concentrations both cell growth inhibition ( P lt ; 0.05 ) . 2 , licofelone and NS -398 TE - 1 cells COX-2 mRNA and protein expression in both time and concentration-dependent inhibition of 5 - LOX and protein time , concentration-dependent expression the inhibition found only in licofelone treated . 3 , NS - 398 and licofelone PGE2 concentration were concentration - and time - dependent inhibition significant difference ( P lt ; 0.05 ) compared with the control group . Levels of LTB4 in the licofelone group was significantly inhibited , and NS - 398 group than the control , and in addition to to 100μM outside groups and the control group was not statistically significant ( P gt ; 0.05 ) TE - 1 cells of 4,100 μM licofelone and 50μM NS-398 for 24h , respectively, 67.1% , 63.8% in G0/G1 phase , 16.8% , 17.3% in S phase , compared with the control group, a significant difference ( P lt ; 0.05), 100μM NS-398 on cell cycle had no effect ( P gt ; 0.05 ) Conclusion: while inhibiting COX / LOX pathways available to the stability of the ESCC cell proliferation inhibition , and single selective COX-2 inhibition in certain concentrations it may promote cell proliferation ; selective COX-2 inhibitor NS-398 at higher concentration , abnormal activation of the LOX pathway , thereby offsetting its inhibitory effect on cell proliferation .

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CLC: > Medicine, health > Oncology > Gastrointestinal Cancer > Esophageal tumors
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