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Characteristics of the Methane Oxidation Coupled to Denitrification in the Aged Refuse and the Landfill Cover Soil

Author: ZhongZhuangMin
Tutor: YinHua
School: Jinan University
Course: Environmental Science
Keywords: Methane oxidation Denitrification Coupled reaction The aged refuse The landfillcover soil
CLC: X75
Type: Master's thesis
Year: 2013
Downloads: 27
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Abstract


Landfill gas and leachate are the main secondary pollution from landfill. Especially, themethane from landfill gas and nitrogen from leachate are extremely concerned. At present,biotechnology is the most economical and effective engineering measures for the control ofCH4emissions. The work investigated the characteristics of methane oxidation coupled todenitrification （AME-D） in the aged refuse and landfill cover soil. Firstly, the batch assays wereperformed to investigate the effects of different factors such as CH4, O2and NO3--N withdifferent concentrations on AME-D and thus the optimal reaction condition was obtained bydetermining the gas contents in the samples. Afterwars, the effects of moisture content, particlesize and Cu2+concentration on AME-D were observed in the optimal condition, also the thereaction kinetics was done. Finally, the mechanisms AME-D happened in aged refuse andlandfill cover soil were preliminarily explored by means of isotope tracer and biolog test. Themain results are as follows:（1） The concentrations of CH4, O2and NO3--N have significant influences on the oxidation ofCH4（P <0.01） both in the aged refuse and landfill cover soil, and follow the orders ofCH4>O2>NO3--N. In the aged refuse, when the concentrations of CH4and O2arerespectively10%and20%, the oxidation efficiency of CH4reach to97.77%, the production ofN2is more than11.5%and N2O is less than0.2%. While in the landfill cover soil, when theconcentrationgs of CH4and O2both are20%, those of CH4, N2and N2O are repectively78.5%,more than11.3%and below detection.（2） The most appropriate moisture content for AME-D in the aged refuse and landfill coversoil are respectively33.3%and15.3%, the optimal particle size are both below0.9mm. In theaged refuse,when Cu2+concentrations range from50to100mg.kg-1or400to600mg.kg-1,though the production of N2decreased with the increase of Cu2+, the removal of CH4is higher.In contrast, the higher Cu2+concentration (400600mg.kg-1) was more favorable to AME-D inthe landfill cover soil.（3） Both in the aged refuse and landfill cover soil, the efficiency of methane oxidation isclosely related to microbial activity, the higher microbial activity leads to higher methane oxidation efficiency. The organisms in the aged refuse and landfill cover soil both utilized wellamino acid. The data from isotope tracer suggested that nitrate could stimulate themethane-oxidizing bacteria oxiding much CH4and producing much intermediates，and a certainconcentration of nitrate could promote much NO3--N redoxed into other forms of N instead ofN2in the aged refuse and landfill cover soil.

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CLC: > Environmental science, safety science > Processing and comprehensive utilization of waste > Mining, metallurgical industry,waste disposal and comprehensive utilization
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