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Objective To study the severe preeclampsia serum matrix metalloproteinase -9 (matrix metalloproteinase-9, MMP-9), neutrophil gelatinase-associated lipocalin (neutrophilgelatinase-assoeiated lipocalin, NGAL) concentration and placental tissue MMP -9, NGAL mRNA expression and preeclampsia, and to explore the possible role of MMP-9 and NGAL in preeclampsia pathophysiology. By enzyme-linked immunosorbent assay (ELISA) detection of patients with severe pre-eclampsia (cases: early-onset group of 30 patients, 30 cases of late-onset group), normal late pregnant women (control group: gestational age ≤ 34 weeks the control group of 30 patients automatic biochemistry analyzer, gestational age gt; 34-week control group of 30 patients) of serum MMP-9, NGAL and level of MMP-9/NGAL complexes; lipids, creatinine (Cr) and uric acid (UA) levels. Using real-time fluorescence quantitative polymerase chain reaction (real-time PCR) to detect cases and control groups placental tissue in MMP-9, NGAL mRNA quantitative expression levels. Results 1. Cases of serum MMP-9, NGAL water average higher than the same period in the control group, and early-onset group than in the late-onset group, the difference was statistically significant (all P lt; 0.05). 2 cases of serum total cholesterol (TC), triglyceride (TG), low density lipoprotein (LDL), apolipoprotein B (ApoB) level and Cr, UA levels were higher than the control group, serum high density lipoprotein (HDL), apolipoprotein A1 (ApoA1) were lower than the same period in the control group, the difference was statistically significant (all P lt; 0.05). 3 cases of serum MMP-9 and NGAL, MMP-9/NGAL complexes, TG, LDL, ApoB, systolic pressure (SP), diastolic blood pressure (DP), mean arterial pressure (MAP) were positively related; HDL was negative correlation, the difference was statistically significant (P lt; 0.05). 4 cases serum NGAL and MMP-9, of MMP-9/NGAL complexes, LDL, Cr, UA, 24h urine protein, SP, DP, MAP showed a positive correlation; was negatively correlated with HDL, the difference was statistically significant ( both P lt; 0.05). The case group placental tissue in MMP-9, NGAL mRNA expression levels were lower than the control group, and the early-onset group than in the late-onset group, the difference was statistically significant (all P lt; 0.05). 6 NGAL mRNA expression of of water average MMP-9 mRNA expression levels were positively correlated in the case and control groups placental tissue, the difference was statistically significant (all P lt; 0.05). 7 case and control groups placental tissue in MMP-9 mRNA, NGAL mRNA expression levels of serum MMP-9, NGAL level had no correlation (P gt; 0.05) Conclusion 1. Preeclampsia serum MMP-9, NGAL level rise and placental tissue MMP-9, NGAL mRNA expression levels of decline may be associated with pre-eclampsia, especially related to early onset preeclampsia, suggesting that two may be involved in the pathophysiology of preeclampsia process. (2) the preeclampsia patients with serum MMP-9, NGAL level significantly increased vascular endothelial cell injury trigger may be involved in the pathophysiology of preeclampsia. 3 patients with preeclampsia placental tissue in MMP-9, NGAL mRNA expression levels decline, it may be one of the reasons that lead to impaired trophoblastic invasion.
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