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Fundamenal Study on the Engraftment Kinetics in Double Unrelated Cord Blood Stem Cell Transplantation

Author: ZhouZuo
Tutor: SunZiMin
School: Anhui Medical University,
Course: Internal Medicine
Keywords: Double cord blood Implanted dynamics CD34 cells In vitro co-culture Differentiation Proliferation
CLC: R733
Type: Master's thesis
Year: 2010
Downloads: 12
Quote: 0
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Abstract


Background and Purpose of cord blood hematopoietic stem cells are hematopoietic stem cells (haematopoietic stem cell, HSC) sources, the HSC number of cord blood contains relatively thus limiting its application in adults. Double mixed cord blood transplantation in the treatment of leukemia to be successful, to provoke their mechanism of interaction between. The study confirmed that patients receiving double umbilical cord blood transplantation, the majority of patients with long-term hematopoietic reconstitution only from a cord blood to a cord blood early implantation incurs exclusive, but double umbilical cord blood transplantation implanted kinetic mechanism is inconclusive, suggesting that the double cord blood lymphocytes and the generation of the advantages of cord blood related. Investigate double unrelated cord blood transplantation for hematologic malignancies implanted law of dynamics and clinical efficacy. Experiments will double cord blood CD34 cells and CD3 cells mixed culture, observe whether the impact of CD3 cell proliferation and differentiation of CD34 cells. 21 cases of malignant blood system in patients with malignant diseases were unrelated double umbilical cord blood transplantation method clinically observed object, using the multiplex amplification of fluorescently labeled short tandem repeat (STR-PCR) combined with capillary electrophoresis, dynamic testing DUCBT recipients after transplantation of cord blood engraftment, Statistics and implantation may be related to the data. Experiment to establish a liquid and semi-solid culture system, the immune beads election purified double cord blood CD34 cells and CD3 cells were mixed cultured for 6 days and 14 days. CD34 cell culture differentiation indicators observed to flow cytometry (CD33, CD41, CD71); counting colony-forming units (GM-CFU, BFU-E, GEMM-CFU) analysis of CD34 cell proliferation. 1 18/21 cases of umbilical cord blood transplantation engraftment DUCBT hematopoietic reconstitution, absolute neutrophil count (ANC) gt; 0.5 × 109 / L, the median time after transplantation 20 (14 to 35) days, platelet GT; 20 × 109 / L, the median time after transplantation 34.5 (25 to 49) days. Displayed by the STR-PCR detected 17/18 patients affected by the formation of the advantages of a single cord blood implanted. Non-dominant parts of umbilical cord blood infusion, the total number of nucleated cells (total nucleated cell, TNC), the number of CD34 + cells and CD3 cells was 2.17 × 107 NC / kg (0.96-3.98 × 107 NC / kg), 1.16 × 105CD34 / kg (0.36-2.70 × 105 CD34 / Kg) and 1.71 × 106CD3 / Kg (0.40 - 10.65 × 106 CD3 / kg), the advantage of these two parts of umbilical cord blood were 2.34 × 107 NC / Kg (1.87-4.45 × 107NC/kg ), 1.15 × 105CD34 / Kg (0.08-4.00 × 105CD34 / Kg) and 3.225 × 106 CD3 / Kg (0.51-13.92 × 106CD3 / kg, the difference was not statistically significant (P = 0.718,0.936,0.073) 2 liquid after co-culture of each of the CD34 cell surface differentiation index changes cord blood CD34 cell the selected enriched purity (98.5 ± 0.92)%. 3 days experimental group and the control group differentiation was no difference (P GT; 0.05) ; six days CD33, CD71 experimental group was significantly lower than the control group, and CD41 was significantly higher (P lt; 0.05). CD34 cell proliferation changes in the experimental group in the semi-solid co-culture of erythroid colony-forming units (BFU-E) and granulocyte set of single-cell colony-forming units (GM-CFU) lower than the control group (P lt; 0.05), mixed cell colony formation (GEMM-CFU), no difference with the control group (P GT; 0.05) Conclusion 1 double umbilical cord blood transplantation is safe and effective, broad application prospects. TNC and CD3 cells may be related with the generation of the advantages of cord blood; two cord blood CD34 cells and CD3 cells in mixed cultures of CD34 affect cell proliferation and differentiation capacity, suggesting that the adoption of the double cord blood can be part of the interaction between the CD3 cell-mediated.

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CLC: > Medicine, health > Oncology > Hematopoietic and lymphoid neoplasms
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