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Construction and Function Evaluation of Novel Biochip Dialyzer

Author: LiJiWei
Tutor: ZhuWen
School: Huazhong University of Science and Technology
Course: Materials Science
Keywords: HK-2cell HUVEC cell microfuidic chip dialyzer filtration capability physiological function
CLC: TH789
Type: Master's thesis
Year: 2013
Downloads: 7
Quote: 0
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Abstract

Now, the disease’s incidence rates have increased rapidly in the world, more and morepatients suffer from paining of end-stage renal disease due to they can’t accept the welltreatment. Treatment of end-stage renal disease patients by renal transplant is hindered bya shortage of donor organs and most of the patients should accept hematodialysistreatment. But this treatment is very expensive, inconvenient, and only play a filtrationfunction, it does not perform the reabsorption, excretion, metabolic and endocrinefunctions of renal tubules, and then causing a serious complications. Although experts ofdifference countries are studying the bioartificial renal tubule assist device in the present,and obtained certain result. But this device is based on the traditional dialyser, defect ofhollow fiber pipe lead to cells cannot live a long time in the dialyzer, and also thesedialysers are easy to be blocked. Therefore, it is very important to research a more suitabledialyzer for biodialysis.With the development of technology, microfluidic technology has more and moreapplication in biological. We fabricate a micro channels chip by wire electrode cuttingmethod in this topic research, and this kind of structure chip has small size effect, it couldbe dialysis under laminar flow and simple boost control. So it can speed up the substancetransfer rate in the process of dialysis, reduce the blood pressure and the loop bloodvolume of dialysis, and then reduce the volume of the dialyzer. We assembling dialysersby Polyether Sulfoneand, Mixed Cellulose Ester, Regenerated Cellulose respectively withthe micro channels chips, we have conducted a series of studies on dialyser combine thecell engineering and change the number of chips.The Human renal proximal tubular epithelial cells （HK-2） and Human vascularendothelial cells （HUVEC） were culture on three type of dialysis membrane respectively.The cell viability and proliferation on three types membrane were examined by MTT, andresearch the influence of cell activity under dialysis environment. Stain the cells nucleiwith Hoechst33342and investigated the cells’ morphology on the membrane by fluorescence microscope, and considering the influence of morphology under shear force.And the morphologies of two cells on the PES surface were observed using filed emissionscaning electron microscope （SEM）. After obtained the optimal growing states of cells onthe membrane surface, construct biomembrane, assembling biochip dialysers, evaluate theanticoagulant function of dialyzer by recalcification time method. Comparative study onthe clearance rate of UN, phosphate and vitamin B12of several types of dialyzer.Evaluation the absorption function of dialyzer by sodium and potassium reabsorption. Toevaluate the physiological function of the multilayered biochips dialyzer, both theendocrine function and the excrete ammonia function were measured. In addition, as asubsequent work, to evaluate the physiological function of TiO₂nanotubes biomembrane.Results show that two kinds of cells have a preferential growth on the PES membranesurface, nuclear normal, round and cytoplasmic clear, and cell activity was better underdialysis environment. And there was gathered phenomenon on the Mixed Cellulose Ester,the activity of cells is very small on the Regenerated Cellulose surface. Biomembrane withculture cell has very good anticoagulant function, it could dialysis without heparin. Theleakages of six layer chip dialyzer was the best, and the dialyzer of the PES as dialysismembrane has the best effect. And also, biochip dialyzer can perform the renal tubulesfunction of reabsorption, metabolic and endocrine functions. This illustrated themultilayered biochips dialyzer has a good effect, and pave the way to develop artificialdialysis system with multi-function.

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