Dissertation > Excellent graduate degree dissertation topics show

Studies on the ATPase of the Diamondback Moth Plutella Xylostella(L.)(Lepidoptera Plutellidae)and Its Inhibiors

Author: LinZuoZuo
Tutor: ZhouXiaoMao
School: Hunan Agricultural University
Course: Pesticides
Keywords: Plutellaxylostella ATPase inhibitor gene expression
CLC: S433.4
Type: Master's thesis
Year: 2013
Downloads: 5
Quote: 0
Read: Download Dissertation


The adenosine triphosphatase, referred to as ATP synthase (ATPase), was widely found in different organisms, such as microorganisms plants and animals. ATPase could catalyze ATP hydrolyzing to ADP and Pi. The hydrolysis released energy which was used to control the different ion concentration of inside and outside neurilemma, The ATPase could also catalyze ADP and Pi synthetizing to ATP. It was one of the most important enzymes in insect, and involved in major physiological functions of the insects, such as controlling conduction of the nerve system, providing energy for absorption of amino acids. The research on enzyme of ATPase from insects was important to pest control. In this paper we have done research on the properties of the ATPase and the effects of some effectors on its activity from diamondback moth P. Xylostella(L.) by conventional methods in the laboratory. The results was summarized as follow:1. The activity of ATPase from P. Xylostella(L) in different stages and instars was different. The activity of total ATPase was gradual increased with the larval instar from second to forth. And the enzyme activity of the4th instar larva was the highest, which was17.236U/mgprot. However it decreased in the pupal period, while two-day pupae was the lowest, with a degree of8.554U/mgprot.2. The properties of ATPase showed that the optimum value pH for determination of ATPase activity was7.4and the optimum temperature was37℃.The Michaelis constant (Km) of ATPase hydrolyzing ATP was0.151mmol/L and the maximum velocities(Vmax) hydrolyzing ATP was0.676μmol/(mg-h).3. The effects of some metal ions on the total ATPase activity was studied. The results showed that Mg2+、Mn2+、Fe2+could inhibit the ATPase activity. When the concentration was ranged from1.25X10-4~20×10-4mol/L, the inhibition rate was fluctuated between5.67%-77.58%.While K+increased the activity at the low concentration (1.25×10-4mol/L), the enzyme was in the strongest activation which was18.55%.4. The effects of some organic solvents on the total ATPase activity was studied. The results showed that ethanol, acetone, dimethylbenzene and ethyl acetate had inhibitory effects on the enzyme activity. When the organic solvent volume was400μL, the inhibition rate were1.83%,1.62%,6.36%and4.07%respectively. When the organic solvent volume was2000μL, the rate were separately85.06%,51.29%,47.74%and100%. The inhibition of enzyme activity was significantly increased with the increasing of the organic solvent volume.5. By the studying of nine insecticides on the inhibition of ATPase activity, the results showed that pyrethrin (46.99%) was the highest under the same concentration and the second was organophosphorus(profenofos and chlorpyrifos) which was46.01%and45.46%respectively. The following was the carbamate (fenobucarb and methomyl) with rates of44.62%and34.46%seperately. The percentage of inhibition of.ATPase activity by remaining insecticides including imidaclprid, thiamethoxam, dinotefuran and spinosad was all less than21%. The inhibition of ATPase activity by seven insecticides was affected with the concentration changed.6. The differences of P. Xylostella(L.)V-ATPase β subunit gene expression were determined with real time PCR technology. The results showed that the gene expression was different in different developmental stages of P. Xylostella and the expression of adult stage was the highest and the pupae was lowest, which was2.29and0.68folds respectively compared to the1st instar larvae.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. The Expression Survey of BMP Signalling Pathway in the Human Embryonic Tooth Germ,R78
  3. Effects of Enzymes on Metabolism of Polycyclic Aromatic Hydrocarbons in Plant,X173
  4. Coparative Analyses of Carbon and Nitrogen Metabolism of Flue-cured Tobacco Growing in Henan and Yunnan,S572
  5. Study of Chicken Δ~6 Fatty Acid Desaturase Gene’s Promotor Region Polymorphism and Gene Expression,S831
  6. Molecular Cloning, Prokaryotic Expression and Characterization of a Novel Trypsin Inhibitor in the Frog, Lepidobatrachus Laevis,Q78
  7. Studies on Extracting Mitochondrial DNA and Gene Expressions in Brassica Napus L,S565.4
  8. Functional Characterization of Wheat Na~+ /H~+ Antiporter TaNHX2 and Study of TaNHX2 C- Terminus Domain,S512.1
  9. The Deposition Rule and Gene Expression Involved in Yolk Cholesterol in Different Layer Breeds,S831
  10. Study on the mRNA Expression Level and the Polymorphism of Follicle-Stimulating Hormone Receptor Gene in Taihu Pig’s Ovary,S828
  11. The Effects of Don on Proliferation, Differentiation, and Apoptosis of Chondrocytes in Chicken,S858.31
  12. Expression of WW Domain Containing Oxidoreductase Gene in Cholangiocarcinoma and Its Effect on the Biological Behavior of Cancer Cell Line QBC939,R735.8
  13. Research on Feature Selection and Construction in Emotion Speech Recognition,TP18
  14. Purification and Some Properties of α-Amylase Inhibitor from Avena Nuda,S512.6
  15. Study on Effects of Exogenous Auxin on Fructose Metabolism in Tomato Fruits,S641.2
  16. Effect of Single and Double Mutations of Yeast H3K79L、H4K20L on the Function of ELP3 and Expression of SSA3,Q75
  17. The Regulatory Role of Proliferation and Apoptosis of ERK1/2 and JNK Signaling Pathways in Eight Liver Cell Types during Rat Liver Regeneration,Q418
  18. Effects of Losartan on Na~+, K~+-ATPase in Proximal Tubules Epithelial Cells of Spontaneously Hypertensive Rats,R544.1
  19. Relationship and Significance of Gene Transcription Profiles in Rat Liver Regeneration and Liver Cirrhosis Occurrence,R575.2
  20. The Relationship between Aciduric Virulence Factor F-ATPase Expression in Biofilms and Caries,R781.1
  21. LEF-1 Expression of the (Like) Osteoblast under Shear Forces,R783.5

CLC: > Agricultural Sciences > Plant Protection > Pest and Disease Control > Plant pest and its control > Lepidoptera pests
© 2012 www.DissertationTopic.Net  Mobile