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Cloning and Expression Analysis of Two Candidate Genes for Tassel Size in Maize

Author: ChenLing
Tutor: FuFengLing
School: Sichuan Agricultural University
Course: Biochemistry and Molecular Biology
Keywords: Differential expression Keratin-associated protein Maize Receptor-likeprotein kinase Tassel size
CLC: S513
Type: Master's thesis
Year: 2013
Downloads: 33
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Abstract


Tassel is the male inflorescence of maize. It usually sheds pollens much more than enough for pollination. The excess pollens consume a lot of energy. In advanced counties, maize breeders usually make effort to improve commercial hybrids for small tassel for higher productivity. Plant breeders believed that ideal maize should have a relatively small tassel, because it helps to increase ower leaf transmittance and reduce the nutrient consumption, so that more energy flow toward production of kernels to increase grain yields. Tassel size becomes an indirect selection criterion in maize breeding because of its negative correlation with grain yield. Therefore, the study of maize tassel is an important related factor to further increase the yield of maize.In this study, genomic sequences of keratin-associated protein gene KAP5-4and receptor-like protein kinase gene CLV1were amplified from eleven inbred lines of different size tassel, and used for analysis of multiple alignment, open reading frames, domain structures, and single nucleotide polymorphism. Their differential expression in tassel primordial among these inbred lines at the elongation stage of the apical meristem was detected by quantitative real-time PCR, and used for correlation analysis with tassel size. The results showed that:(1) The relative expression amount of gene KAP5-4is positively correlated with number of primary branch(0.77, p<0.01) and dry weight of tassel (0.83, p<0.01).(2) A single nucleotide polymorphism at the2104th base of gene CLV1consists codon GAC encoding acidic aspartic acid at the702nd site of receptor-like protein kinase in five of the inbred lines(ZI330, ZHENG58, Mo17,178,478), and consists codon AAC encoding polar asparagine at the same site in the other six inbred lines(R08, ES40, RP125, DAN340, S37, CHAN G7-2).(3) The relative expression amount of gene CLV1is obvious higher in six inbred lines whose the2104th base of gene CLV1was A than in other five inbred lines. According to the prediction of domain structure, the2104th base of CLV1gene located in an active site for proton acceptor of serine/threonine protein kinase domain of CLV protein. This SNP may influence the CLV1protein activity.(4) The relative expression amount of gene CLV1is negatively correlated with number of primary branch (-0.92, p<0.01) and dry weight of tassel (-0.91, p<0.05) in five inbred lines whose the2104th base of gene CLVI was G, and negatively correlated only with dry weight of tassel (-0.91, p<0.05) within the later six inbred lines.Therefore, it is concluded that genes KAP5-4and CLVI are closely associated with tassel size. Functional DNA markers can be developed for improvement of tassel size after further study confirmed.

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CLC: > Agricultural Sciences > Crop > Cereal crops > Corn ( maize )
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