Dissertation > Excellent graduate degree dissertation topics show

Preparation and Identification of Monoclonal Antibody to E Protein of Duck Tembusu Virus

Author: LinYuan
Tutor: LiuYi
School: Hunan Agricultural University
Course: Preventive Veterinary Medicine
Keywords: Duck Tembusu Virus E protein monoclonal antibody identification
CLC: S858.32
Type: Master's thesis
Year: 2013
Downloads: 9
Quote: 0
Read: Download Dissertation

Abstract


Duck Tembusu virus (DTMUV) is a newly discovered virus that caused a sebere outbreak disease shocked the poultry industrious duck farms with egg-laying reduction, death, and feed uptake decline.DTMUV belongs to the family Flaviviridae, genus Flavivirus, class Mosquito-borne virus, and it has been serologically classified into the Ntaya virus serocomplex, which includes Bagaza virus, Israel turkey meningoencephalitis virus and Ntaya virus.Most of Flaviviridae virus is contagious zoonosis pathogen, Also has been reported that anti-Tembusu virus antibodies has been found in the human body. These indicate that DTMUV could be disastrous for the duck industry and there is a potential threat to public health. Therefore, the systematic study of the virus, develop a fast and reliable diagnostic method is extremely important.The DTMUV genome encodes three structural proteins:capsid (C), membrane (PrM and M) and envelope (E). E protein is conserved structural protein, plays an important role in virus receptor-binding, entry and fusion.The recombinant plasmid pET30a-E was transformed into E. coli Rosetta (DE3) competent cells at a final concentration of0.5mmol/L IPTG,37℃expression of E protein. SDS-PAGE electrophoresis showed that a soluble fusion protein of55ku was expressed. E fusion protein was purified with the6X His Spin Purification kit.4to6-week-old BALB/c mice were immunized with the purified E protein expressed by prokaryotic expression system. Then mouse spleen cells with SP2/0cells were fused by the PEG regent. Meanwhile, positive hybridoma clones were screened by ELISA that use the purified E protein as antigen. After three cycles of subcloning, Consequently, four McAbs, named as LY12, was generated. Western blot and IFA test analysis showed the MAb reacted with DTMUV but no cross reaction to JEV and WNV. The immunoglobulin subtype of the MAb were IgGl.The MAb could be used to establish the monitoring and differential diagnosis method for DTMUV infection.

Related Dissertations

  1. Research on Combinatorial Regulation of Multiple Transcription Factors,Q78
  2. Recovery Whey Soy Protein by Microfiltration and Development of Whey Powder,TQ936.2
  3. Temperature Drift Modeling and Compensation of Fiber-Optic Gyroscopes,V241.5
  4. Simulation and Analysis about Switched Reluctance Generator Power Supply System,TM31
  5. Research on Vector Control System of Induction Motor Based on DSP,TM346
  6. Research on Disease Related miRNA Mining Based on Protein-Protein Interaction Network,R341
  7. Compensation Methods of Different Speech Coding for Speaker Recognition,TN912.34
  8. The Software Component Modeling Method Based on Feature and Its Application in VMI Management System,TP311.52
  9. The Research and Implementation of Protein Classification Algorithm on the Basic of String Kernel,TP301.6
  10. The Study of Domain Dictionary Construction Based on Web,TP391.1
  11. Research on the Control and Remedy of Nanopositioning Stage Drived by Piezo Material Lead Zirconate Titanate,TP273
  12. Study of Oridonin on SGC-7901 Cell Proliferation by Inhibitting Cell Cycle Proteins,R285
  13. Expression of hBMP4 and hBMP7 in Chinese Hamster Ovary Cells,Q78
  14. The Study of Color Change and Control Technology for Oyster Protein Beverage,TS254.4
  15. Study on Bispecific Monoclonal Antibody to Pesticide Imidacloprid and Parathion-Methyl,S482.2
  16. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  17. Serum A-FABP Levels and Its Expression in Atherosclerotic Plaques of Diabetes Mellitus,R587.1
  18. Impact on Drosophila Brain Developement Through the Overexpression and Loss of Function of Gsα,Q75
  19. Study on Deodorization Techniques of Oyster Protein Beverage,TS254.4
  20. Preliminary Study of Phosphorylation Effect on UGT1A3 Metabolism Activity Towards Drugs,R346
  21. Study on Preparation and Properties of Protein Isolates from Tilapia,TS254.4

CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Poultry > Duck
© 2012 www.DissertationTopic.Net  Mobile