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Research on Central Neural Mechanism of Acupuncture Antagonism to Amblyopia Deprivation Effect

Author: LiTie
Tutor: WangFuChun
School: Changchun University of Traditional Chinese Medicine
Course: Acupuncture and Massage
Keywords: Acupuncture visual cortex amblyopia deprivation effect neuronsspike nNOS GAP43
CLC: R245
Type: PhD thesis
Year: 2013
Downloads: 4
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Abstract


Purpose:Based on the fore-working on the mechanism of acupuncture in the treatmentof amblyopia,from the visual angle of physiology and molecular biology of central cortex,from two aspects of function and morphology, further reveal the central mechanism ofdeprivation effect of acupuncture against amblyopia,in order to improve the clinical curativeeffect of acupuncture in the treatment of amblyopia,provide objective experimental basis toexplore its mechanism of action.Method:Through the establishment of the classic monocular visual deprivation model,by suturing one eyelid of the rat,divided into blank grou(pNG),monocular deprivatio(nMD)group,early of critical period acupuncture group(EG),middle of critical period acupuncturegroup(MG)and late of critical period acupuncture group(LG) of5groups,and accordingto the theory of “the eyes can see because of the blood”in traditional Chinese medicine."Jingming","Cuanzhu","Fengchi"and "Guangming" four acupoints are selected to completethe treatment for7days. Using the technology of multi-channel recording methods forcentralneural activities in vivo,All the rats in the five groups, the amplitude, dischargingfrequency and inter spike interval (ISI) were collected and analyzed; at the same timeusing laser scanning confocal microscopy(Olymbus FV1000),observe and analyze the effectof each animal model of visual neurons the expression of the protein nNOS and GAP-43.Result:1. Compared with model group and blank control group,each rat visual cortical neurons,neurons discharge amplitude average discharge times had extremely significant difference(P<0.01),neuronal firing frequency histogram and ISI to be collected in the channelnumber, the discharge frequency,and the state has undergone pathological obvious change.2,Comparise to the EG,MG and MD group,amplitude,average discharge times ofvisual cortical neurons,were statistically significant (P<0.01,P<0.05),the EG comparedwith the MG,LG,was significant difference (P<0.05). The EG,MG of neurons firingfrequency histogram and the ISI has improved obviously to be collected in the channelnumber,the frequency of discharge,and the payment of state etc.3. P-VEP in MD rats replication after1week,the MD P-VEP waveform changes significantly,the performance of the P100appear as obvious delay,N45-P100amplitudedecreased significantly,compared with the normal group of waveforms were significantlydifferent(P <0.01);4.The BG,nNOS positive neurons in the rat visual cortex area17has a scattereddistribution in,much as non-pyramidal neurons,cell morphology is bipolar,multipolarspindle or triangle,protruding obvious,some dendrites extending and recurrent branch,of thenuclei are not colored fluorescent immune group,compared with MD and BG thefluorescence intensity,weak,decrease in density,small somata,dendritic length is short,there was a significant difference between the mean fluorescence intensity(P<0.01).4W age,5W,6W period of BG and MD of visual cortex area17nNOS immunoreactive cell averagefluorescence intensity compared,the difference was significan(tP <0.01),compared to theEG of4weeks group and MG,the difference was significant(P <0.01),MG compared withMD,significant difference(P<0.05)the LG compared with MD,there was no significantdifference (P>0.05);EG compared with the BG,no significant difference (P>0.05);compared with MG, LG at the MG and BG,there were significant differences (P<0.05).5.BG,GAP-43immunoreactive neurons in the rat visual cortex distribution area17layers Ⅱ~Ⅵ scattered,which distributes in Ⅱ/Ⅲ most layer,followed by IV,V and VIlayer,cell morphology,GAP-43positive substance gathered in cell bodies and axons, nervefiber network is also seen some positive the reactant; compared with the BG,MD, thefluorescence intensity is weak,density decreased, cells are irregular in shape,size is differ,there was significant difference between the mean fluorescence intensity(P<0.01)positivecells in BG and MD of visual cortex area17Ⅱ/ⅢGAP-43immunoreactive cell averagefluorescence intensity compared,there is significant differences(P<0.01),the EG comparedwith the MD,the difference was significant(P <0.01),compared to the MG and MD,therewas significant difference(P<0.05),compared with the LG and MD,no significant difference(P>0.05).Conclusion:1,Based on the“the eyes can see because of the blood” theory in traditional Chinesemedicine,acupuncture "Jingming","Cuanzhu","Fengchi"and "Guangming" four acupointsfor treatment of amblyopia in clinical trials in the past proved to be an effective treatment method, the experiment from the angle of experiment and confirmed the antagonistic effectof Acupuncture on monocular deprivation effect of amblyopia,provides the experimentalbasis for clinical research.2. Acupuncture can be benign regulation of amblyopia model of visual critical period indifferent periods as the central neurons,this effect may be space-time coded cortex neurons inas independent discharge through improved to achieve, its intervention in earlier time,effect is more obvious,and in the final stage of sensitive intervention effect not obviously,which indicated that acupuncture treatment of amblyopia should intervene as soon aspossible,in order to achieve better therapeutic effect.3. Acupuncture can effectively increase the expression of nNOS and GAP-43,monoculardeprivation effect suggested that acupuncture can promote the normal development ofneurons and promote the recovery of injured neurons to antagonize the amblyopia.

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