Dissertation > Excellent graduate degree dissertation topics show

The Observation on Effect of Traditional Chinese Medicine Gumibao Treating Steroid-induced Necrosis of Femoral Head

Author: JiWanBo
Tutor: JiangHong
School: Nanjing University of Traditional Chinese Medicine
Course: TCM orthopedics and traumatology
Keywords: Steroid-induced Avascular Necrosis of the Femoral Head Traditional Chinesemedicine Gumibao Lipid Metabolism Bone Metabolism Biomechanics
CLC: R274.9
Type: Master's thesis
Year: 2013
Downloads: 15
Quote: 0
Read: Download Dissertation

Abstract


Objective:The purpose of this study is to establish an animal model with clinical pathological characteristics, the disorder of lipid metabolism, intravascular coagulation, bone disorders, osteoporosis in the steroid-induced avascular necrosis of the femoral head in the pathological course of action. And focuses on eliminate the Stagnant and Activate the Blood, Invigorate the Kidney and Replenish Qi-the Effect of Chinese Medical Herb Gumibao on steroid-induced avascular necrosis of the femoral head model of rat femoral head bone tissue morphology, ultrastructure, lipid metabolism, angiogenesis, bone resorption, bone formation effect. To explore the mechanism and efficacy of Chinese medicine, to provide scientific theoretical basis for the treatment of this disease.Methods:Two-month-old SD rats were200of clean grade, half male and half female. Randomly selected30rabbits as blank control group. Molding method according to the S. Okazaki et al, the rat tail vein injection of LPS on two consecutive days of2mg/kg, once a day, the last time after injection of LPS, three consecutive days of injection of methylprednisolone20mg/kg, once a day. The last injection of methylprednisolone after2weeks from the blank control group, model group respectively were6, pathological observation, confirmed that the model was successful. Randomly divided into blank control group (group A), model group (group B), western medicine group (Fosamax Group C), the Gumibao of high-dose group (D), bone mineral density by weight based on Celebrexprescription dose group (E), the Gumibao of low-dose group (F), n=8, male and female. The Gumibao of is high, medium and low dose group rats with body surface area conversion the shares secret Celebrex were orally suspension, western medicine group rats with body surface area conversion gavage dose of Fosamax suspension model groupand blank control group were fed with normal saline.4,8and12weeks after operation respectively intervention.By hematoxylin and eosin staining was used to observe the morphology, observation of morphological changes of femoral head cartilage and bone cell toluidine blue staining, to observe the changes in the number of osteoclasts and TRACP staining, transmission electron microscopy ultrastructural observation, observation of angiogenesis, VEGF staining, Micro-CT scan, biomechanical testing, CHOL, TGL, HDL, LDL detection of lipid metabolism, PICP, CTX-I,1,25-(OH) to detect the level of-D in bone metabolism.Results:The animal model of animal in the injection after LPS died of anaphylactic shock in17, Gumibao group gavage killed during3, western medicine group by gavage killed during2,2rats died in model group and spontaneous.HE staining showed that the trabecular bone of the model group sparse arrangement, shape tapering gradually continuity interruption, bone marrow tissue steatosis, bone karyopyknosis, while poly, stained bone marrow edema, visible fat emboli, a large number of lacunaevacuoles.Blank control group with normal morphology of the femoral head. Western medicine group with normal trabecular bone structure, less breakage, rare lacunae, bone cell shrinkage, nuclear margination. Gumibao group:the high-dose group trabecular bone morphology slender, still arranged neatly, breaking more, a large number of lacunae, bone karyopyknosis, while poly. In the low dose group, trabecular bone structure was normal, nuclear pyknosis, chromatin is less, the bone lacunae number is less, have rich blood vessels under the cartilagie.Toluidine blue staining showed the model group chondrocytes irregular, disorder, the surface cartilage severe disability dye, epiphyseal line at the matrix uneven dyeing, coloring shallow, the osteoblasts irregular, nuclear condensation, nuclear staining light blue, cytoplasmic staining light, epiphyseal plate vacuoles formed around the trabecular bone cells reduce the number. The western medicine group cartilage surface coloring is deep, cartilage cells arranged in neat rows, settling time clearly, nuclear staining evident. Gumibao group uniform staining of the surface cartilage of the femoral head, clear, deep coloring of the surface of the cartilage, cartilage cells arranged in neat rows, clear Shen times, nuclear staining, the presence of bone cells of empty lacunae, the nucleus margination, condensation, trabecular bonearranged in neat rows, the trabecular part of the fracture.VEGF expression located in the cytoplasm and membrane, the positive was brown. Model group after2weeks femoral head VEGF expression was decreased,4weeks,8weeks, although the expression of VEGF within12weeks of the femoral head elevated, but not yet returned to normal levels. Blank control group, VEGF expression did not change. Gumibao group after4weeks of drug intervention based found weak expression of VEGF in the femoral head, the enhanced expression of VEGF in the femoral head within8weeks,12weeks, gradually returned to normal levels.TEM ultrastructure observation:blank control group, bone cells, cartilage cells with normal morphology. The model group appeared in the cytoplasm of lipid droplets, and gradually increase, full of cytoplasm; bone cell nuclear pyknosis, chromatin; bone cell calcification; nuclear and cytoplasmic edema radiolucent zone; trabecular fold, has obvious calcification adhesive line and the newly formed bone; intravascular red cell overlapping even a "Min line", closely arranged, thrombosis.TRACP staining shows, model group TRAP-positive multinucleated cells increased significantly. Around the western medicine group subtidal and trabecular scattered osteoclasts multiple irregular nucleus in a mature or immature shape. Gumibao groups subtidal and trabecular bone scattered around the more mature or immature multicore irregular osteoclast cells. Micro-CT bone morphogenetic3D analysis shows that the model group, BMD, BMC, TMC, TMD, BV/TV to reduce, Tb.SP, SMI, BS/BV increases, share the same Gumibao group, middle dose group compared statistically difference (P<0.05), with Gumibao of the low-dose group and western medicine group difference was significant difference (P<0.01), shares close Celebrex prescription, low-dose group compared with the western medicine group was no significant difference (P>0.05). Micro-CT scan showed normal development of the femoral head of the control group. The rough surface of the femoral head of the model group, the gradual emergence of cellulite degeneration the, early epiphyseal cartilage District cystic change, in the late subchondral bone collapse. The western medicine group and bone trabecula was sparse, the thickness of different sizes, arranged neatly, consistent with the line of force direction. Gumibao groups femoral head surface is rough, stock density remains low dosage group of femoral head bone a little subsidence, epiphyseal plate better continuity, the trabecula was sparse, arranged neatly, consistent with the line of force direction.Serum TC, TG, HDL, LDL shows, model group, TC, TG, HDL was significantly higher HDL reduced, with the other groups was statistically significant (P<0.05). Gumibao groups compared with the western medicine group has no statistically significant difference (P>0.05).High levels of serum PICP content and western medicine group, the Gumibao groups of high-dose and low-dose group were significant difference (P<0.001). The Gumibao groups of high-dose group content of serum PICP with the western medicine group was not statistically significant (P>0.05). The model group CTX-I content is the highest, with the control group, the Gumibao groups of high, middle dose group and western medicine group were statistically significant (P<0.05), the Gumibao groups of low-dose group CTX-I content than western medicine group (P<0.05); model group1,25-(OH)-D content was the lowest, with the blank control group, a statistically significant difference (P<0.05), and western medicine group difference was statistically significant (P<0.001), and there is a significant difference (P<0.01) in the high dose of Gumibao groups.Biomechanical test results showed that the Gumibao groups femoral head subchondral bone maximum load, stress intensity, axial stiffness, elastic modulus significantly better than the model group (P<0.001), Gumibao groups femoral headsubchondral bone displacement values than the model group, two more significant difference (P<0.001). Femoral shaft three-point bending test shares Gumibao groups of various biomechanical indicators are better than the model group (P<0.01).Conclusions:The disorder of lipid metabolism, angiogenesis, reduce the number of bone metabolism imbalance, osteoporosis may be the pathogenesis of steroid induced necrosis of femoral head. Gumibao Fang can regulate lipid metabolism, promote the formation of new blood vessels, accelerate bone repair, inhibit bone resorption, improving biomechanics, delay the collapse of femoral head.

Related Dissertations

  1. Effects of Creatine Pyruvate on Lipid and Protein Metabolism in Broiler Chickens,S831.5
  2. Effect of Maternal Dietary Protein Level on Fat Deposition in the Piglets at Weaning and the Research of Lipid Metabolism Regulation Mediated by Leptin,S828
  3. The Study of the Effects of Isoflavones of Puerarin and Daidzein on Bone Metabolism in Ovariectomized Female Rats,R580
  4. The Establishment and Preliminary Application of the Three-dimensional Finite Element Model of Ankle Joint,R687.3
  5. The Biomechanics and Imageology Preliminary Studies of Lumbar Intertransverse Absorbable Fusion Cage in the Process of Biodegradation,R687.3
  6. Biomechanical Comparison and Clinical Analysis of Three Different Fixation Methods for Postermedial Tibial Plateau Fracture,R687.3
  7. Effect of Conjugated Linoleic Acid on Gene Expression of Lipogenesis and Lipolysis in Liver of Mouse,S816
  8. Effects of 60Coγ Radiation Exposure on Expressions of CDKN1A, FABP4 and HSL in Human Lymphocytoblast,R144
  9. The Study of the Mechanism and the Influence of Osteogenesis Activity by Hepcidin on Osteoblast,R580
  10. Gait Analysis and Dynamic Research on Biped Walking Robot,TP242
  11. The Study of Triggering Autorhythmicity of Rat Aortic Ring and Its Mechanism and PMQ Ameliorated Glucose and Lipid Metabolic Disorders and Vascular Endothelial Dysfunction in GK Rats,R96
  12. Shandong Province 64kg men's boxers before Zhiquan Biomechanical analysis technique,G886.1
  13. Walking Biomechanical analysis and risk of injury,G821
  14. Effect Onproduction Performance,Egg Quality and Feed Digestable Ratio of Layers with Adding Cellulase to Alfalfa Meal Ration,S831.5
  15. The Establish and Clinical Application of Osteocalcin Test by Chemiluminescence Immunoassay,R914
  16. The Research on the Plantar Pressure Comfort of High-heeled Shoes,TS943.1
  17. The Investigation of the Mechanism of Reno-protective Effect on 3/4 Nephrectomized Rats with Different Protein Diets,R692
  18. Research on the Mechanism of Bone Metabolism Disor-ders of Osteonecrosis of Femoral Head,R681.8
  19. Relationship between 25-(OH)D3 and Disease Activity and Bone Metabolism in Ankylosing Spondylitis,R593.23
  20. The Effect of Different Frequency Impulse Electro Magnetism on OPG Gene Knock-out Mice Which Were Suffered from Osteoporosis,R580
  21. Kazak hyperuricemia and relationship between glucose and lipid metabolism,R589

CLC: > Medicine, health > Chinese Medicine > Journal of Orthopaedics > Modern medicine, orthopedic diseases
© 2012 www.DissertationTopic.Net  Mobile