Dissertation > Excellent graduate degree dissertation topics show

The Change of C3G Protein Expression in the Peri-infarct Myocardium in Rats

Author: WangLiPing
Tutor: LiGang
School: Chongqing Medical University
Course: Geriatrics
Keywords: C3G(Crk SH3domain-binding guanine nucleotide-releasing factor) myocardium myocardial infarction Rat
CLC: R542.22
Type: Master's thesis
Year: 2013
Downloads: 10
Quote: 0
Read: Download Dissertation

Abstract


AIM： To investigate the change of C3G protein expression in theperi-infarct myocardium in rats.Methods：The SD rats were randomly divide into two groups experimentalmyocardial infarction (MI) groups and sham-operation (sham) groups byLitwin method. The rats in the MI and sham groups were sacrificed at24hand12weeks after the operation respectively and the myocardium werecollected: getting the myocardium in the apex (infarct myocardium werecontained in the MI groups),we use the technology of Picric acid-sirius redstaining and immunohistochemistry to test the CVFⅠ、Ⅲ and C3G proteinexpression; The expression of C3G protein in non-infarcted myocardium inthe MI group and in the cardiacbase myocardium in the sham group wasmeasured by Western blotting respectively.Results： The result of Picric acid-sirius red staining is the CVFⅠ、Ⅲ inPost-MI12-week group is14.01±2.73、4.80±0.64, which is increased mostamong the post-M I24h group (3.68±0.95、1.53±0.61), Post-sham24h-group(3.15±0.70、1.42±0.48) and Post-sham12-week group(3.27±0.65、1.47±0.52),(p<0.05),however, there is no any difference in thelatter three groups(all p>0.05). The immunohistochemistry detect that theC3G protein expression in the post-MI12weeks group is significantly 0increased than the other three groups(p<0.05),but the latter three groups haveno difference(all p>0.05). C3G protein expression in the non-infarctmyocardium in the post-MI12weeks group(0.56±0.06,n=8)wassignificantly higher as compared with that in the apex-myocardium in the inthe Post-sham24h-group (0.22±0.04,n=8),in the non-infarct myocardium inthe post-MI24h-group (0.29±0.02,n=8)and Post-sham12-week group (0.22±0.04,n=8) respectively (All P <0.05).Conclusions： C3G protein exists in the myocardium in rats. And In theperi-infarct myocardium, the expression of C3G protein is increasedsignificantly. The increased C3G protein expression could be involved in thepostinfarction cardiac remodeling.

Related Dissertations

  1. The Value of the Heart-Type Fatty Acid Binding Protein in Early Diagnosis of Acute Myocardial Infarction,R542.22
  2. Platelet parameters in patients before and after acute myocardial infarction,R542.22
  3. Comparison of Efficacy and Safety between Facilitated PCI and Primary PCI on ST-segment Elevation Myocardial Infarction,R542.22
  4. Therapeutical Effects of Diammonium Glycyrrhizinate Lipid Ligand on Nonalcoholic Steatohepatitis and Its Partly Mechanisms of Action,R575.5
  5. Effect of Nrf2-ARE Pathway on Isolated Rat Hearts Using Ischemia or Pinacidil Postconditioning,R96
  6. BMP2 and VEGF165 Genes Transfected Rat Bone Marrow Mesechymal Stem Cells Simultaneity Expression,R329
  7. The Affection and Correlation of VEGF、 SDF-1 to the Homing of Bone Marrow Hemopoietic Stem Cells in Myocardial Infraction Area,R542.22
  8. Effects of Receptor Activity Modifying Protein-1 Transplantation of Mesenchymal Stem Cells on Postangioplasty Restenosis and Cardiac Function after Myocardial Infarction in Rabbits,R542.22
  9. Effects of High-salt Dieet on Blood Pressure and the Remodeling of Carotid Arteries and the Intervention of Telmisartan in Wistar Rats,R544.1
  10. Intracoronary Versus Intravenous Administration of GP IIb/IIIa Inhibitor Improves Myocardial Reperfusion in STEMI Patients,R542.22
  11. The Beneficial Effects of Soy Isoflavones in Osteoporosis of Ovariectomized Rats,R580
  12. Morphological Characteristics of Brain Capillary Endothelial Cells and Neurons in a Ticellular System for Study on Neuroprotection and Neuroregeneration in Vitro,R651.1
  13. Dorsolateral rat hypothalamic nucleus 5 - serotonin and 5 - HT 2A receptor expression,R338
  14. Ultrasound technology assessment dimensional strain in patients with acute myocardial infarction in emergency and delay the clinical value of stenting,R542.22
  15. On the presence or absence of previous angina pectoris in patients with acute myocardial infarction risk factors and clinical characteristics of,R542.22
  16. Heart rate variability and coronary artery disease and prognosis of acute myocardial infarction,R541.4
  17. Underwent emergency surgery and PCI in STEMI patients with cardiogenic shock, the clinical characteristics and prognosis,R542.22
  18. Effect of Short-term Atorvastatin Calcium Preconditioning on Matrix Metalloproteinases during Myocardial Reperfusion in Rabbits,R965
  19. The Injury Mechanism of Ethylhexyl Phthalate (DEHP) and Its Metabolites Single-ethylhexyl Phthalate (MEHP) on Spermatoge-nesis in Young Male Wistar Rats,R965
  20. Statistical Inference for Spatial Distribution of Rat Density in Three Gorges Reservoir Before and After Water Impounded,R184
  21. The Related Analysis about QT Interval Variability,HRV and the Occurrence of Ventricular Arrhythmias in Acute Myocardial Infarction,R542.22

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Heart disease > Myocardial diseases > Myocardial infarction
© 2012 www.DissertationTopic.Net  Mobile