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PTEN and AR Expression and Their Correlation Study in the Rat Model with Polycystic Ovary Syndrome

Author: ZuoZuo
Tutor: LiuDongE
School: Central South University
Course: Clinical
Keywords: Polycystic ovary syndrome Rat model hyperandrogenism AR PTEN
CLC: R711.75
Type: Master's thesis
Year: 2013
Downloads: 22
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Abstract


Objective To investigate the expression levels of PTEN and AR in PCOS rat model group and control group, in order to explore the role of PTEN and AR in the pathogenesis of PCOS.Methods30female SD rats of42-days-old were randomly divided into3groups:①the PCOS model group:the rats were administered with letrozole at concentrations of lmg/kg dissolved in1%carboxymethyl Cellulose (CMC) p.o. daily for21days;②the experiment control group: the rats were administered with1%CMC p.o. daily for21days;③the blank control group:the rats receive normal diet for21days.The body weight and the change of oestrous cycle of these rats were observed continuously. Rats were sacrificed at Day22of the experiment, ovarian weight,serum level of LH,FSH,E2,T and INS were determined, and their ovaries were excised to be given ovarian histological detection. Immunohistochemistry and Immunofluorescence were used to detect the localization and expression of PTEN and AR in the rats ovaries, and the correlation of the expression of PTEN, AR and serum hormone level were also studied. Results1. The rats in the PCOS model group manifested higher body weight (P<0.05) and irregularity of oestrous cycle,their ovaries also seemed to be polycystic change with more cystic follicles,less corpus luteum numbers and decreased of granular cell layers. The serum level of E2in the model group was significantly lower than that in the two control groups, while the serum levels of LH, FSH and T were significantly higher (P<0.05). The level of fasting serum insulin has no statistically difference in three groups.2. Immunohistochemistry and immunofluorescence showed that PTEN was mainly expressed in the cytoplasm of granulosa cells in rat ovary, and the expression of AR was seen in the nucleus of granulosa cell,theca cells and stromal cells.The expression of PTEN and AR in granulosa cells were both significantly higher than those in the two control groups (P<0.05). The expression of AR (r=0.609, P=0.000) and PTEN (r=0.747, P=0.747) in the granulosa cells was significantly positively related to the serum testosterone level, and the expression of PTEN and AR also had significant positive correlation (r=0.805, P=0.805).Conclusion1. The letrozole-induced PCOS rat models were similar to those of the patients with PCOS, and the model was successfully established with hyperandrogenism. 2. The high level of serum androgen may increased the expression of AR in granulosa cells in the ovary of the PCOS model rats,and the high levels of expression of PTEN and AR may related to the pathogenesis of PCOS together.

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CLC: > Medicine, health > Obstetrics and Gynaecology > Gynecology > Other diseases of the female genital > Ovarian disease
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