Dissertation > Excellent graduate degree dissertation topics show

Involvement of VPO1in the Endothelial Dysfunction in Hypertension

Author: PengHaiYang
Tutor: ZhangGuoGang
School: Central South University
Course: Clinical
Keywords: Angiotensin Ⅱ vascular peroxidase oxidative stress endothelial dysfunction
CLC: R544.1
Type: Master's thesis
Year: 2013
Downloads: 13
Quote: 0
Read: Download Dissertation

Abstract


OBJECTIVE:To explore the relationship of VPO1expression and the endothelial dysfunction in spontaneously hypertensive rats (SHR) and Ang Ⅱ-induced Human umbilical vein endothelial cells (HUVECs), and the possible mechanism.METHODS:Ten20-week-old male SHRs served as SHR group, and ten age and sex matched Wistar Kyoto rats (WKY) were selected as control group. Endothelial-dependent relaxation of thoracic aorta, aortal VPO1, Nox4, eNOS expression, and VPO1, H2O2, NO in plasma were determined. HUVECs were incubated in Ang Ⅱ, in the presence or absence of Nox-specific inhibitor DPI, H202hydolytic enzyme Catalase, or VPO-specific inhibitor ABAH. Cellular VPO1, Nox4, and eNOS expression were measured, as well as H2O2, HOCl, and NO concentration. Inhibition of HOCl to eNOS was also determined.RESULTS:Aortal VPO1and Nox4expression were increased in SHRs, as well as VPO1and H2O2in plasma. Aortal eNOS expression and NO concentration in plasma of SHRs were decreased, and the endothelial-dependent diastolic function was impaired. In cultured HUVECs, Ang Ⅱ increased expression of VPO1in a concentration and time-dependent manner. Incubation of Ang II elevated level of H2O2and HOCl, and decreased the expression of eNOS and NO production. Treatment of HOCl directly depressed the expression of eNOS. Nox4expression was inhibited by DPI, while H2O2level was decreased by DPI and Catalase. The decreased expression of VPO1by DPI, Catalase and ABAH led to down-regulated HOC1production, which elevated the expression of eNOS and NO production.CONCLUTION:VPO1may play a critical role in the endothelial dysfunction in hypertension. Activated expression of VPO1by Ang II can utilize Nox4-derived H2O2to produce HOCl, which depresses the expression of eNOS, leading to the endothelial dysfunction.

Related Dissertations

  1. Effect of β-Casomorphin7 or Casein Peptides on Blood Glucose and Oxidative Stress of the Diabetic Rats,R587.1
  2. Effect of β-Casomorphin-5 on Blood Glucose and Oxidative Stress of Diabetic Rats,R587.1
  3. The Effects of Aerobic Exercise Training on Oxidative Stress and Ultrastructure of Pancreatic Organization in Diabetic Rats,R587.1
  4. Expression and Role of Uncoupling Protein 2 in the Kidney of Mouse after Ischemia Reperfusion,R587.1
  5. The Mechanism and Role of Cavelion-1 in Oxidative Stress Induced by Intermittent High Glucose in Endothelial Cells,R587.1
  6. The Injury Induced by High Glucose and with the Correlation of SelS and PKC Signal Transduction Pathway,R587.1
  7. The Effects of the Aqueous Extract of Dendrobium Noblie Lindl on the Nonenzymatic Glycation and Oxidative Stress in Diabetic Rat Kidney,R285.5
  8. In Vitro Study of Theeffects of Fullerol on the Hippocampal Synaptic Plastisity,R741
  9. Comperative Experiment on the Toxicity of Particulate Matter from Different Cities,R114
  10. Oxidative stress, heat shock protein 90 pairs of 20S proteasome function,R363
  11. Effect of Purple Sweet Potato Flavonids on Metabolism of Glucose, Lipids and Oxidative Stress in Diabetic Rats,R285.5
  12. Protective Effects and Mechanism of N-acetylcysteine on Diaphragma in COPD Rats,R563.9
  13. Effects of Distribution of Body Fat and Lipid-regulating Drugs on Coronary Heart Disease,R541.4
  14. Experimental Research of Myocardial Mitochondrial Injuries in Septic Rats,R459.7
  15. Oxidative stress markers in the serum of patients with endometriosis and follicular fluid expression and role,R711.71
  16. The Study of Interventional Effect of Jiaweitianmagou-tengyin Decoction on the Early Hypertensive Renal Damage and Vascular Endothelial Dysfunction of SHR,R285.5
  17. Studies on Submerged Fermentation Culture and Hypoglycemic Activity of Selenium-enriched Mycelia of Coprinus Comatus,S567.39
  18. The Effect of Angiotensin Ⅱ on Choriocarcinoma BeWo Cell Line in Secreting sFlt-1,R714.246
  19. The Study of Probucol as Antioxidant in the Rat Brain,R965
  20. ARMS2 Iocalization and Expression in Retina Tissue and Cells,R774.1
  21. The Study of Development and Cell Cycle Checkpoint Response of Early Embryos in Vitro Fertilized with DNA-damaged Sperm Induced by Oxidative Stress,R714.8

CLC: > Medicine, health > Internal Medicine > Heart, blood vessels ( circulatory ) disease > Abnormal blood pressure > Hypertension
© 2012 www.DissertationTopic.Net  Mobile