Dissertation > Excellent graduate degree dissertation topics show

Expermenlal Study of VEGF、Sugical Delay on the Survival Rate of the Subdermal Vascular Network Skin Gratf

Author: LiuJun
Tutor: ZhuJingMin
School: Dalian Medical University
Course: Surgery
Keywords: Subdermal vascular network skin graft delay surgery survival rate of VEGF adrenomedullin
CLC: R654.3
Type: Master's thesis
Year: 2013
Downloads: 6
Quote: 0
Read: Download Dissertation

Abstract


Objective: To explore whether the surgical delay of the subdermal vascular networkskin graft and subdermal injection of VEGF can improve the survival rate of skingraft;2. Observed the diameter and cross-sectional changes after the operation ofthe subdermal vascular network;3.Discussing the number changes in the averagemicrovascular using the growth factor (VEGF) and the roll played by theadrenomedullin (ADM) in the mechanism of formation of subcutaneous bloodvessels in the skin graft delay.Method： Rats with subdermal vascular network skin graft delay model,intradermal injection of VEGF model, and the superposition model were created toobserve the changes of survival rate，blood vessel diameter and the cross sectional areaafter the skin graft; The delay model skin graft within the organization VEGF andADM qualitative and quantitative analysis were carried out using the enzyme-linkedimmunosorbent assay (ELISA)in the organization of skin delay model to detectwhether the changes of the expression of ADM and the VEGF in the skin graft surgerywere correlated with the changes of subdermal vascular network using the statisticalanalysis.Result：1.The survival rate of growth factor group,the the delayed group and theoverlay group was significantly higher than that of the saline group (P <0.05);there is no significant difference between the delayed group and the growth factorgroup(P>0.05). The superimposed group were significantly different than that ofother experimental group (P <0.05)；2、Comparing with the base value,the levels ofVEGF in the delay model after3days skin graft increased significantly but sigifcantlyhigher than that of7/14d,(P <0.05)and no significant differences of14days with thebase values.3、The expression of ADMmRNA were analysised using the RT-PCR inthe wound tissue and detected using the double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) in the peripheral blood and the wound tissue.Theresults shows that there was no significant difference with the chip average vesseldiameter(MVD) of corresponding delay time interval.Conclusion:1、Intradermal inection of VEGF on Rat can promote local vascularendothelial growth, with the biological effects of promoting the formation of newgranulation;2.Both the Model of subcutaneous injection of vascular endothelial growthfactor (VEGF) and skin graft delay can effectively improve the survival rate of the skingrafts of subdermal vascular network, but the mechanism of the two wasdifferentgrowth factor (VEGF) can further improve the survival rate of skingraft;3.Compared with the saline control group, the skin graft with subdermal vascularnetwork has a higher survival rate;It came to the conclusion that the VEGF played animportant role in t he delaying process of skin graft and the process ofvascularization;3.The results suggest that although there are changes of ADMmRNAexpression in the flap tissue after the graft trauma in two weeks, the content of theperipheral blood and Local tissue flap were detected by enzyme-linked immunoassay(ELISA) shows no significant changes of the ADM concentration and to a certainextent that there is no significant correlation with vascularization of subdermal vascularnetwork.

Related Dissertations

  1. Plasma Adrenomedullin in Relevant to Obstructive Sleep Apnea-hypopnea Syndrome and Hypertension,R544.1
  2. The Effect of ADM on Endothelial Progenitor Cells from the Patients with Stable Angina Pectoris,R541.4
  3. To Compare the Effects of Apoptosis of Adrenomedullin Gene and Hepatocyte Growth Factor Gene Transfection on Bone Marrow Mesenchymal Stem Cells Under Hypoixa,R542.22
  4. The Protective Effect of Recombinant ADM in Skin Flap Ischemia Reperfusion,R965
  5. The Study of the Activated Pancreatic Stellate Cells on the Invasion and Metastasis in Pancreatic Carcinoma,R735.9
  6. Clinical Study on Relationship among Lipid Metabolism and Adrenomedulin in Deficiency of Spleen and Kidney, Profuse of Sputum Phlegm Syndrome of Senile Metabolische Syndrom,R259
  7. Morphine-induced Neurochemical Alterations in Rat DRG Neurons,R965
  8. The Expression and Correlation of ADM and VEGF in Plasmas and Tissues of Patients with Lung Cancer and Their Clinical Significance,R734.2
  9. Adrenomedullin Gene Transfected Bone Marrow Mesenchymal Stem Reduce Apoptosis under Hypoxia-cultured Conditions,R329
  10. Mechanism and Modulation of Morphine Tolerance-Role of Adrenomedullin and Effect of SNSR Agonist BAM8-22,R96
  11. Mechanisms of Chronic Pain-Expressions of Adrenomedullin and Bovine Adrenal Medulla 22,R402
  12. Anatomic Basis of Humeral Neck Flap and Its Clinical Application,R622
  13. Adrenomedullin and coronary heart disease and different types of relationship between the number of coronary vessel disease,R541.4
  14. Adrenomedullin (ADM) induced by hypoxia in rat proximal tubular epithelial cell apoptosis and its mechanism,R965
  15. Adrenomedullin on the intestinal microvascular endothelial cell injury in septic rats,R459.7
  16. Effect of adrenomedullin on Tubular Cell Apoptosis Induced by Renal Ischemia Reperfusion Injury and Its Mechanisms,R692
  17. Hypoxia-inducible factor 1α (HIF-1α) and adrenomedullin (ADM) in preeclampsia placenta, Expression and significance of plasma,R714.24
  18. Adrenomedullin in the kidney tumor tissue expression and microvessel density correlation,R737.11
  19. The Expression of Adrenomedulin and Adrenomedulin Receptor in Kidney Carcinoma Patients,R737.11
  20. The Peripheral Revascularization Establishment Research of Intermittent Hypobaric Hypoxia Preconditioning on the Subcutaneous Vascular Net Free Skin,R622
  21. The Relationship between Essential Hypertension with Target Organ Damage and Plasma Adrenomeduelin Levels,R544.1

CLC: > Medicine, health > Surgery > Of surgery > Cardiovascular and lymphatic system surgery > Vascular
© 2012 www.DissertationTopic.Net  Mobile