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In Vitro Study of Sorafenib Combined with Cisplatin Inhibits the Growth of Human Ovarian Cancer H08910Cell Line

Author: HuDingYue
Tutor: ZhangShuang
School: Liaoning Medical
Course: Obstetrics and Gynaecology
Keywords: ovarian cancer sorafenib cisplatin P53 STAT3
CLC: R737.31
Type: Master's thesis
Year: 2013
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Abstract


ObjectiveExplore sorafenib combined with cisplatin on cultured human ovariancancer H0-8910inhibition of cell proliferation and apoptosis and the possiblemechanism of action.MethodsMTT method was used to detect different concentrations of sorafenib,cisplatin single drug and combination of the two drugs on human ovarian cancercell line H08910proliferation inhibition effect. Observed by light microscopegroup of drugs to change the form of the human ovarian cancer H08910cell line,Using Hoechst fluorescent staining under the effect of the drug weremorphological changes of cell apoptosis. Apoptosis and cell cycle blockade wasdetected by flow cytometry. Using Western Blot method to detect P53,STAT3gene protein expression levels.ResultsSorafenib combined with cisplatin on growth inhibition rate than singlemedication group on human ovarian cancer H08910cell line, sorafenib also hadan obvious inhibitory effect on human ovarian cancer H08910cell line. Cellmorphology was observed under light microscope, sorafenib group volumeshrinking shows polygonal cells, cell membrane shrinkage, the decrease in thenumber of cells, bad to stick wall, combined treatment group were more obviouschange. Hoechst fluorescent dye has obvious nuclear pyknosis and other typical apoptosis changes, the combination group than the medicine groupobviously. Flow cytometry showed sorafenib block the cell cycle of ovariancancer cell line H08910in G0/G1, can make the cell apoptosis, the combinationgroup of G0/G1and G2/M phase cells were increased, cell apoptosis was alsosignificantly. Western Blot assay results showed that sorafenib and sorafenibcombined with cisplatin group, can increase the P53protein expression leveldecreased the expression of STAT3protein level, between groups wassignificant difference (P <0.05).ConclusionSorafenib synergy with cisplatin on inhibiting and promoting apoptosis hasobvious effect on proliferation of human ovarian cancer H08910cell line, Itsmechanism may be to the drug can inhibit the RAF signaling pathways,directinhibition of tumor growth; And also can inhibit VEGFR and blocking theformation of tumor angiogenesis, indirectly restrain the growth of the tumor; canincrease the P53protein expression level decreased the expression of STAT3protein level, induced cell cycle arrest and apoptosis related.

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CLC: > Medicine, health > Oncology > Genitourinary tumors > Female genital tumors > Ovarian tumors
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