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Inhibitory Effects of Recombinant Human Endostatin on Oxygen-induced Retinal Neovasculariztion in Mouse

Author: BaiZuoZhou
Tutor: ZhaoJiaLiang
School: Beijing Union Medical College
Course: Clinical
Keywords: retinal neovascularization endostatin intravatreal injection
CLC: R774.1
Type: PhD thesis
Year: 2010
Downloads: 15
Quote: 0
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Abstract


Objective:To study the effects of intravitreal injection and intraperitoneal injection of recombinant human endostatin (rh-ES) on retinal neovascularization in an oxygen-induced model of retinopathy in mouseMethods:7-day-old C57BL/6mice were put back to room air after exposed to75%oxygen for5days.0.04μl(0.2μg) rh-ES(0.2μg)/Bevacizumab(1.0μg)/NS were injected into vitreous to the eyes of intervention group/positive control group/negative control group, respectively. After another5days of room air exposure, mice were sacrificed to evaluate the morphological changes of retinal vessels by FITC-dextran perfusion and retinal whole mount. Count the number of the nuclei of the endothelial cell breaking through the internal limiting membrane (ILM) under microscope to compare the inhibitory effects of each group on retinal neovascularization. Another2groups with intraperitoneal injection of rh-ES (15μg) and N.S.0.1ml respectively, were set to evaluate the effects of IP injection.Results:Compared to oxygen-exposed group, the branches of retinal vessels went normal with less un-perfused area in rh-ES group. The number of nuclei of endothelial cells breaking through ILM on retinal cross-section decreased to (23.0±1.55) per slide, as in the oxygen-exposed group was (54.6±3.10) per slide (P<0.01), and in IP injection group, the number was (12.0±1.59) per slide, much less than in negative control group (58.2±3.78)) per slide.Conclusion:Intravitreal injection of rh-ES can effectively inhibit the formation of retinal neovascularization in mice. IP injection is also comparatively effective, which reveals systemic administration may be a possible way of treatment.

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CLC: > Medicine, health > Ophthalmology > Retina and optic nerve diseases > Retinal disease
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