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Construction of a New Portable Fluorescence Detector

Author: HeZuo
Tutor: DuWei
School: Huazhong University of Science and Technology
Course: Biomedical Engineering
Keywords: POCT Microfluidic Fluorescence detector
CLC: R318.6
Type: Master's thesis
Year: 2013
Downloads: 11
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Abstract


The properties of low cost, easy to use and short detection time make the POCT (Point-of-care testing) be extensively developed in recent years. With the discovery of the structure of DNA and elucidation of the molecular mechanisms of genetics, PCR has become a powerful method in diagnosis in genetic and infectious disease. PCR microfluidic chip have attracted considerable attention in POCT due to advantages of miniaturization, integration, high-throughput and shorter reaction time. But the large volume of the detection system makes micro-scale PCR not suitable for POCT. So it is very interesting to develop a portable fluorescence detector for the microfluidic chip.In this work, two micro-optical systems were built with high-power LED as the excitation source. System I was an opposite-type optical system, which included an objective lens to collect excitation light and a PMT for photoelectric conversion. Three important parameters including fluorescence collection angle, detection distance and LED driven current were investigated to improve SNR (Signal to Noise Ratio) of the system. The detection limits (S/N=3) were5X10-8mol/L for sodium fluorescein and10-7mg/mL for DNA, respectively. System II got a smaller and cheaper optical system by changing the structure, using a small-angle high-power LED, ordinary lens and a PIN photodiode. The size was30mm×20mm×20mm and the qualitative detection rate of sodium fluorescein was100%.Circuit design mainly included the power supply module, the LED drive module, the signal processing module and the micro-controller module. This product uesed ARM microprocessor STM32F101as a core processor, that integrate an A/D conversion and data acquisition, a human-machine interface (Button control and LCD display), output control (Output instruction for LED driver, Stepper motor driver, Indicated LED), and a bus interface to realize the correspondence function.The fluorescence detector investigated in this thesis had the advantages of miniaturization, automation and high throughput capability, which could be effectively used in microfluidics-based POCT products.

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CLC: > Medicine, health > Basic Medical > Medical science in general > Biomedical Engineering > Apparatus, equipment
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