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Study on the Influence of Acidiphilium Cryptum on Differential Expression of the As3+ Resistance Gene and Bioleaching Efficiency of Acidithiobacillus Ferrooxidans

Author: LiLi
Tutor: XiaJinLan
School: Central South University
Course: Biological Engineering
Keywords: Acidophilic Thiobacillus ferrooxidans Hide acidophilus The arsenic resistant gene arsH Real - time quantitative PCR Cu2 As3
CLC: Q93
Type: Master's thesis
Year: 2010
Downloads: 66
Quote: 0
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Abstract


Acidophilic Thiobacillus ferrooxidans (Acidithiobacillus ferrooxidans) is an important leaching bacteria, is also the most studied one of the bioleaching bacteria. Acidiphilium cryptum of a leaching auxiliary bacteria, it can restore Fe (Ⅲ) and using A. ferrooxidans synthesis of organic compounds. In this way, it can eliminate the inhibitory and toxic effects of these organic and A. ferrooxidans Fe (Ⅱ) as an energy source. As the two strains were grown in acid mine water, special and harsh environment, and therefore able to tolerate high concentrations of heavy metals such as copper, arsenic. Acidiphilium cryptumDX1-1 at the same time to promote the growth of A. ferrooxidans whether transcriptional expression of the of A. ferrooxidans arsenic resistance gene also have some impact is the focus of this study. In this paper, the two strains A. ferrooxidans the CMS and Acidiphilium cryptumDXl-1 Dui Cu2, As3 two heavy metal ions highest tolerance. The results showed that the highest tolerated concentration of the CMS bacteria of Cu2 higher than DX1-1 bacteria DX1-1 bacteria of the As3 the maximum tolerated concentration higher than the CMS bacteria. Experiments show that the the ferrous oxidation activity and As3 resistance DX1-1 bacteria join CMS bacteria have a significant role in promoting. The the bioleaching efficiency under the strains of A. ferrooxidans the CMS and Acidiphilium of heavy metal stress cryptumDX1-1 alone and mixed role of bioleaching results found DX1-1 bacteria leaching less effective, but it can significantly improve the CMS bacteria. The addition of heavy metal ions causes leaching rate, and the higher the concentration of heavy metal ions, the leaching rate is lower. Through Real-timePCR study the differential expression of the anti of arsenic gene arsH A. ferrooxidans ATCC23270 different arsenic environment Acidiphilium cryptumDX1-1. The results show that the the arsenic resistant gene arsH of the As3 the stress is more sensitive and is likely to play an important role in the mechanism of bacterial arsenic resistance. Cultured alone, with the As3 increase in the concentration of growth environment the ATCC23270 strain arsH expression are raised. The higher the concentration of arsenic ions, raised more obvious. ATCC23270 strain and DX1-1 strain of the mixed culture, and cultured alone is more similar, with the increase of the concentration of the growth environment As3 the ATCC23270 strain arsH gene expression raised. The higher the concentration of arsenic ions, raised more obvious. Raised multiples lower than the upregulation of multiple the ATCC23270 strain under the same arsenic ion concentration cultured alone. DX1-1 strain of adding a certain extent, affected the the ATCC23270 strain arsH gene transcriptional expression. Bioinformatics analysis showed that horizontal gene transfer occurs not only between strains of different species of the same outline also occur between different species Gang. Dilution curve environment, there are still a lot containing arsenic resistant bacteria arsH genes yet to be discovered. arsH gene on microbial arsenic resistance plays an important role in the mechanism of action, however, still needs further research proved. Simultaneous analysis of a wide range of special features found containing arsenic resistant bacteria in arsH genes, including pathogen infection, plant symbiotic decomposition of polymers, the production of chlorophyll and resistance to heavy metal. Collectively, these results indicate that the bacterial arsenic resistance, the the arsenic resistance Gene arsH in play an important role, Acidiphilium cryptumDX1-1 can promote the growth of A. ferrooxidans, while improving the arsenic resistance. These studies further study of arsenic resistance the gene arsH the function, and Acidiphilium cryptumDX1-1 laid the foundation for the interaction with A. ferrooxidans.

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CLC: > Biological Sciences > Microbiology
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