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New temporary metal stents canine cardiac achalasia experimental study

Author: KuangXiaoChun
Tutor: ChengYingSheng
School: Suzhou University
Course: Medical Imaging and Nuclear Medicine
Keywords: Achalasia Esophagus BAC Dog Animal Model Stand
CLC: R571
Type: Master's thesis
Year: 2010
Downloads: 14
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Abstract

The first part of the canine achalasia model Objective: To establish a canine model of achalasia, for the new temporary cardia metal stents achalasia experimental study conditions. Methods: 24 adult beagle dogs, under endoscopic guidance tetradecyl - dimethyl - benzyl ammonium chloride (benzyl-dimethyltetradecylammonium chloride, BAC, 4 mmol / L, 12 mL) was injected into the lower esophageal circumferential sphincter (lower esophageal sphincter, LES). BAC was observed after the clinical manifestations of experimental animals. BAC drug treatment before and after 6 mon respectively esophageal manometry and timing of esophageal barium (timed barium esophagogram, TBE), to evaluate the success of modeling. Results: BAC before and after treatment in dogs LES resting pressure has a very significant statistical difference (22.88 ± 3.13 mmHg vs 47.14 ± 6.12 mmHg; P lt; 0.001). TBE dogs before processing the first 5 min of barium display width, height, and the product was 0.96 ± 0.55 cm, 3.87 ± 2.19 cm and 4.55 ± 3.74 cm2, respectively, after treatment 3.04 ± 0.47 cm, 9.74 ± 1.33 cm and 29.39 ± 4.35 cm2, between the two groups there was a significant statistical difference (both P lt; 0.001). After eating to reduce the incidence of 62.5% (15/24), returning food (vomiting) occurred in 50% (12/24), weight loss rate was 54.2% (13/24). Experimental animals did not occur during the experiment deaths. Conclusion: Endoscopic BAC LES circumferential injection drugs can create an ideal achalasia canine model. The second part of the new temporary metal stent treatment of canine cardiac achalasia results Objective: To explore new temporary metal stent treatment of canine cardiac achalasia efficacy and complications, follow-up study stent after removing different time points esophageal histopathological changes characteristic segment. MATERIALS AND METHODS: cardiac injection tetradecyl - dimethyl - benzyl ammonium chloride (benzyl-dimethyltetradecylammonium chloride, BAC, 4 mmol / L, 12 mL), on 24 experimental Beagle dogs achalasia disease modeling work, a total of 24 successful modeling and randomly divided into control group (n = 12), the experimental group (n = 12), respectively, normal esophageal stent placement and new temporary cardiac stent; indwelling stent removed after four days . In modeling before and after modeling stent after removing 1 wk, 1 mon, 3 mon and 6 mon, respectively esophageal manometry and timing of esophageal barium (timed barium esophagogram, TBE), and the stent after removing each observation time point were sacrificed three dogs, feeding Road cardia segment for histopathological examination. Results: The stent insertion / removal and follow-up examinations were successfully implemented. The two groups did not occur esophageal perforation and severe esophageal tear. Compared with the control group, the experimental group a lower incidence of stent migration (experimental group 1 patients; control group, 5 cases), the experimental group, the lower esophageal sphincter (lower esophageal sphincter, LES) pressure is reduced more significantly (after removing 6 mon with stent significant differences between the groups, p = 0.027), the experimental group TBE 0 ~ 5 min barium column multiplied Poor greater (when the stent after removing 3 mon with significant differences between the groups, P = 0.009). Mouse anti - PCNA monoclonal antibody immunohistochemical staining showed the epithelium were hyperplastic inflammation peaked within 2-4 wk, no statistically significant difference between the groups. Mouse anti-α-SMA monoclonal antibody immunohistochemical staining showed submucosal smooth muscle proliferation were reached peak within 2-4 wk, no statistically significant difference between the groups. Masson trichrome staining showed submucosal collagen fibers proliferative activity gradually increased and stabilized at 3 mon state when no statistical difference between the groups. Conclusion: In this study, a new temporary treatment of canine cardiac stent is safe and effective for achalasia, esophageal stents more effective than ordinary, but the sample size needs to be larger and longer follow-up time of basic experimental and clinical research. An appropriate time for stent placement issues need to be further explored. With other observed variables proposed TBE compared using TBE 0 ~ 5 min \

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