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Purpose preliminary isolated and cultured rat corpus cavernosum cells detect stem cell markers Stem Cell Antigen -1 (Sca-1), Oct4 and myogenic markers desmin (Desmin) expression, to explore whether the existence within the corpus cavernosum derived stem cells and muscle-derived stem cells. Methods 2-month-old SD rats, weight 200 ~~ 250g, ether inhalation anesthesia, under sterile conditions in corpus cavernosum tissue type Ⅰ collagenase, trypsin digested tissue until the cells in the presence of basic single-cell morphology , filtered, and collected by centrifugation, the cell pellet was resuspended culture, containing 20 ℅ FBS DMEM. Using a modified Preplate differential adherent initial isolation and culture of cells, PP1-PP6 adherent cells obtained by flow cytometry, immunofluorescence cytochemistry method Sca-1 Western blot detection observed isolated cells, Oct4 and Desmin expression line Sca-1/Oct4, Sca-1/Desmin, Oct4/Desmin immunofluorescence double labeling. The isolation and culture lessons PP1 and PP2 cells adherent fast the long fusiform fibroblast-like cells, the PP3-PP5 cell adherent ability gradual slow down, short spindle cells, and some were polygonal vascular endothelial cells, smooth muscle cells mainly PP6 cells are smaller, into circular floating cells, this very slow adherent cells 2-3 days after adherent round or spindle. Flow cytometry: PP3 cells Sca-1, Oct4 positive expression were 0.3 ℅ ± 0.2 ℅ ℅ 0.2 ℅ ± 0.3, compared with the same type of control group were not statistically different (p> 0.05), Desmin positive The expression level of 15.3 ℅ ± 1.5 ℅, a statistically significant difference (p <0.05) compared with control group; PP6 cells Sca-1, Oct4, Desmin positive expression for 5.7 ℅ ± of 0.7 ℅ 2.6 ℅ ± 0.3 ℅, 41.1 ℅ ± 1.6 ℅, were statistically significant (p <0.05) compared with PP3. Immunofluorescence cytochemistry PP6 in a small number of cells expressing Sca-1 and Oct4 are scattered, Sca-1 cytoplasmic expression-based, Oct4 expression in the nucleus; expression Desmin cell aggregation in larger quantities, mainly also found that a very small amount of double-labeled cells (Sca-1/Oct4, Sca-1/Desmin, and Oct4/Desmin) expression in the cytoplasm; expression in the cytoplasm and nucleus of the same cell Sca-1/Oct4, Sca- expressed in the cytoplasm of the same cells 1/Desmin the, Oct4/Desmin expressed in the nucleus and cytoplasm of the same cells. Western blot showed that the Sca-1 and the Oct4 protein is not detected in the PP1-PP5 cells significant expression, but to the Sca-1 and the expression of the Oct4 protein is detected in the PP6 cells, Desmin protein expression increased in the PP1-PP6 cells. Conclusions found in rat corpus cavernosum cells with stem cells and muscle-derived stem cell phenotype, suggesting the presence of endogenous stem cells and muscle-derived stem cells in the corpus cavernosum.
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