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Nucleotide is possibly involved in many important physiological processes, is wildly used in the Agriculture, Food industry and Medical industry. Adenosine 5’-monophosphate (5’-AMP) is one of the Nucleotides, was found effective in blocking the perception of bitterness. Therefore 5’-AMP may be used as flavor enhancers in foods and pharmaceuticals, which has broad prospects.This research used adenosine and phosphoric pentoxide as two main materials, succeeded in preparating 5’-AMP, used the technology of recrystallisation to purify the crude product, then analyzed 5’-AMP’improvement in sense of taste.In the reaction of direct phosphorylation, the effects of the dosage of triethyl phosphate, the molar ratio of the material, the quantity of the catalyst, reaction temperature, reaction time on the yield of 5’-AMP were studied, and the result of orthogonal experiment showed that the optimum reaction condition was: the dosage of triethyl phosphate was 36mL, n(adenosine):n(phosphoric pentoxide)was1:2.8, n(adenosine):n(catalyst) was1:0.7, reaction temperature was 20℃,reaction time was 6h, and the yield of 5’-AMP was 73.40%.In the recrystallization process for isolation and purification 5’-AMP, the effects of the volume ratio of water to ethanol, the dosage of mixed solvents, the dosage of activated carbon, stirring temperature, stirring time on crystallization yield of 5’-AMP were studied, and the result of orthogonal experiment showed that the optimum recrystallization condition was: the volume ratio of water to ethanol was1:3, the dosage of mixed solvents was 150mL, the dosage of activated carbon was 2% of 5’-AMP, stirring temperature was 55℃,reaction time was 30min, and the crystallizationyield and the purity of 5’-AMP were 88.05% and95.68%.In the research of flavor improvement, found that 5’-AMP did not alter the behavioral responses to sourness, sweetness, saltness, but could inhabited bitterness. Compared withγ-aminobutyric acid, Ferulic, Zinc Lactate, 5’-AMP could effective in blocking the perception of Quinine Hydrochloride’bitterness with low-doses but appreciate flavors. Meanwhile 5’-AMP could inhabit artificial sweeteners including Saccharin Sodium, Acesulfame K’off-bitterness, but no obvious inhibitory effect of Stevioside’off-bitterness, only slightly improved.
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