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Have an important regulator of TGF-β 1 (Transforming growth factorβ 1 , TGF-β 1 ) on cell growth , differentiation, and immune function role in normal physiological pregnancy process , TGF -β 1 maintain normal during pregnancy , embryo implantation , placental development and fetal growth has an important role to play an important role . For the to explore transforming growth factor β1 on the gestation period of the rat uterus and placenta RT1-A and RT1-DM expression of this experimental use of the The rats injected supraphysiological doses Spreqne-Dawloey TGF -β 1 using RT-PCR and Real -time PCR and Western blot detection of TGF-β 1 RT1-A and RT1-DM rat uterus and placenta at mRNA and protein levels , and by immunohistochemistry (IHC) its express positioning . The results show that : the RT-PCR and Western blot results showed that (1) TGF -β the 1 compared to the rat uterus RT1-AmRNA expression levels and protein levels of the control group after treatment has increased , and preimplantation stage (P <0.01), the implantation period ( P <0.01 ) , and implanted late ( P <0.001 ) , and late pregnancy was significantly different (P <0.01) ; ( 2 ) in the second trimester , TGF-β 1 treated RT1-A protein expression was reduced , while in the the late pregnancy TGF - β 1 treated RT1-A protein relative expression level , and a significant difference (P <0.01 ) ; ( 3 ) during early pregnancy in rats rat uterus RT1-DM mRNA expression levels and protein levels rise, the decline in mid-gestation , its expression levels in late pregnancy , late in the preimplantation stage (P <0.05), implanted ( P <0.001) and late pregnancy has a significant difference (P <0.05) ; (4 ) mid-gestation TGF-β 1 treated RT1-A protein expression was no significant difference in the amount of the control group , while treated the late pregnancy TGF -β in 1 RT1-A protein relative expression level , and there is a significant difference ( P <0.01 ) ; ( 5 ) in the maternal-fetal interface RT1-A and RT1-DM distributed in the glandular epithelium , perivascular , luminal epithelium and maze layer , compared with the control group , no significant changes in expression of both parts . The experimental results show that : the TGF -β 1 pregnant rat uterus and placenta RT1-A and RT1-DM expression has a certain role , and this role has a time -specific .
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