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The Study of the Characteristic and Key Factors of Simultaneous Desulfurization and Denitrification under Different Inoculum Conditions

Author: HouShengChun
Tutor: XuJinLan;HuangTingLin
School: Xi'an University of Architecture and Technology
Course: Environmental Engineering
Keywords: Sync desulfurization and denitrification Inoculum Microbial properties Sulfur and nitrogen than HRT
CLC: X703
Type: Master's thesis
Year: 2009
Downloads: 45
Quote: 0
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Abstract


The test anaerobic sludge Thiobacillus denitrificans bacterial suspension and anaerobic sludge and add nitrogen and Thiobacillus bacterial suspension to the inoculum start synchronization desulfurization and denitrification reactor, research reactor under different inoculum the desulphurization and denitrification characteristics, microbial properties and produce sulfur granules characteristic; anaerobic synchronization desulfurization system and to study the impact of sulfur and nitrogen ratio, organic matter, and HRT and other factors. An inoculum of anaerobic synchronization desulfurization and denitrification characteristics: (1) the inoculum has a larger impact on the synchronization desulfurization and denitrification characteristics anaerobic sludge denitrification Thiobacillus bacterial suspension mixture inoculum enhanced anaerobic sludge system has the best the desulfurization features and denitrification characteristics of sulfide and nitrate removal rate is close to 100%; while anaerobic sludge inoculum reactor has strong denitrification nitrate removal efficiency of the system is always maintained at about 100%; bacteria suspension of Thiobacillus denitrificans inoculum reactor desulfurization ability. (2) different inoculum under the conditions of each reactor denitrification sulfur removal capacity large differences, mainly because the inoculum was not the same, is formed in the reactor system the advantages of different microbial communities. Inoculated with anaerobic sludge # 1 reactor, mainly cocci vaccination isolated from denitrification sludge the pure bacterium Thiobacillus denitrificans # 2 reactor, main oval tuberculosis, 3 # reactor inoculated with anaerobic sludge at the same time add Thiobacillus denitrificans bacterium to form a complex microbial communities spherical, oval and short rod-dominant bacteria, nitrate removal stable performance of sulfur. (3) through the scanning electron microscope observation reactor to produce elemental sulfur tablets, different characteristics of each reactor the sulfur generated pellets. Inoculation of anaerobic sludge # 1 generated by the reactor of the sulfur particle size of 0.5μm or so, the generated sulfur granules difficult in conjunction with the system of bacteria, mostly separated from the environment of the system; inoculated denitrification Thiobacillus strengthening anaerobic 3 # generate the sulfur particle size about 0.2μm, the smaller particle size, and the majority is attached to the bacterial surface. 2, sulfur and nitrogen than anaerobic synchronized desulfurization and denitrification characteristics: (1) strengthen the system of anaerobic sludge and pure culture of Thiobacillus denitrificans system desulfurization and sulfur helium in unrelated 24h average sulfide removal efficiency of up to 97%, and the anaerobic sludge system is gradually reduced as diltiazem ratio increase its sulfide removal the best Sulphonitrocarburizing ratio of 5/2. (2) to strengthen the capacity of the anaerobic sludge denitrification system from water sulfur and nitrogen than the limit, the nitrate removal rate was higher in each sulfonitriding than the conditions under anaerobic sludge system with the sulfur nitrogen ratio increases denitrification capacity enhanced, thus a high S / N ratio is conducive to the removal of nitrate; pure strain of Thiobacillus denitrificans system ratio by diltiazem, diltiazem ratio of 5/3, 5 / 2,5 / 1 conditions, the nitrate removal rate is only about 20% of the nitrate in the water when the S / N ratio increased to 5/5, not detected. 3, the retention time on the anaerobic synchronized desulfurization and denitrification characteristics: (1) the determination of the different periods of water can be clearly seen the sulfides in 3h basically all removal with sulfur and nitrogen than nothing to do, and nitrate in 3h removal of about 11.57%, diltiazem ratio of 5/1, 5/2, 5/3, 5/4, 5/5, under the condition, the removal of nitrate per mmol sulfide consumption amount, respectively The theoretically generated 0.03,0.13,0.19,0.20,0.28 mmol, the amount of elemental sulfur 1.77,2.13,1.79,1.91,1.60 mmol. (2) between 3h to 6h, 65% of the nitrate nitrogen is converted, the system sulfide concentration is very low, indicating that the sulfide may not restore the electron donor is nitrate. In 73% of the sulfide is converted to elemental sulfur at 3h, results that strengthen the elemental sulfur 3h anaerobic sludge system restore nitrate electron donor. Nitrate removal load increased with increasing nitrate concentration. Nitrate and sulfide reaction first produced nitrite, nitrite generation influent S / N ratio, When the influent S / N ratio of 5/1 and 5/2, 3h reaction nitrosyl The nitrogen concentration accumulated up to 30mg / L, reaction 6h after the accumulation of nitrite disappeared; For influent S / N ratio of 5/3, 5/4 and 5/5 reaction bottles, nitrite, respectively, the maximum cumulative concentration 62.6mg / L, 45.6mg / L and 93.66mg / L, 21h after the water was not detected nitrite. 4, the influence of the organic matter of the anaerobic synchronized desulfurization and denitrification characteristics: (1) less affected by the anaerobic sludge system, the concentration of organic matter from 200mg / L increased to 775mg / L, add and add, sulfide go removal rate is substantially no change; pure strain of Thiobacillus denitrificans system and enhanced anaerobic system, when the organic COD concentration is higher than 427.59mg / L, sulfide removal dropped significantly. When COD dosage greater than 427.59mg / L, the removal rate began to decline. (2) organic material impact on the pure strain of Thiobacillus denitrificans system nitrate removal larger the organics concentration of higher than 452.25mg / L the denitrification ability to inhibit was inhibited. Add organic matter can increase the anaerobic sludge system and strengthen the system of anaerobic sludge nitrate removal efficiency, COD to higher than 609.57mg / L inhibited denitrification capacity.

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