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A general increase in the standard of living today, diabetes has become the third largest killer of a threat to human health from cardiovascular diseases and malignant tumors. The upward trend in its incidence, has become a common disease. The etiology and pathogenesis of diabetes has still not very clear, and no cure for the disease of drug clinical. Single targets of Western medicine treatment of diabetes, and adverse reactions, has a long history of traditional Chinese medicine in treating diabetes, non-toxic side effects of long-term use. Mulberry Moraceae Mulberry Morus alba L. dried leaves, since ancient times, there used to only diabetes, documented that modern diabetes. In recent years, the hypoglycemic activity attracted widespread attention and research role. Hypoglycemic effects of substances in mulberry leaves, the Task Force has conducted a systematic study, the efficacy of hypoglycemic component polysaccharides, flavonoids and alkaloids in. The use of modern extraction separation and preparation of technical papers on the basis of preliminary studies on mulberry hypoglycemic efficacy component separation and purification and formulation research, developed oral formulations of the treatment of diabetes - Mulberry hypoglycemic capsule system studied Mulberry hypoglycemic capsule formulations molding process, quality standards, stability, and preliminary pharmacodynamics. Main contents and conclusions are as follows: Extraction and purification of polysaccharide from mulberry leaves. Using single factor and orthogonal experiments, the rate of the indexes polysaccharide from mulberry leaves, preferably the best extraction process: extraction solvent is purified water, solid-liquid ratio 1:15 times, 90 ° C reflow extracted twice, each time 100min. The combined extracts were concentrated, and precipitated with 50% ethanol, the mulberry total polysaccharide MP1 are. MP11, MP12, MP13 three polysaccharides MP1 was further purified by DEAE-cellulose column and Sephadex G-100 column chromatography. By means of chromatographic and chemical analysis methods, and analysis of the monosaccharide composition of the three components of MP11 is mainly composed of rhamnose, arabinose, xylose, mannose, glucose and galactose, the molar ratio of 21:16:3 : 3:1:20; MP12 mainly composed of rhamnose and glucose composition, the molar ratio of 3:1; the MP13 main rhamnose. Extraction and purification of mulberry leaves flavonoids. The single factor and orthogonal experiments, total flavonoids was investigated indicators, preferably optimum extraction process of 70% ethanol, 20 times the amount of crude drugs, 85 ° C extracted twice, each time 2h. A purity of 37.1% of the total flavonoids yield was 28.1mg / g. Macroporous resin purification of total flavonoids process. As index to the the total flavonoids adsorption amount, screening four macroporous adsorption resin, S-8 resin adsorption and desorption best performance. Static adsorption performance of the S-8 type resin, the resin adsorbed amount of total flavonoids 31.62mg / g wet resin, desorption rate was 82.69%. And to study the dynamic adsorption properties of the resin, the resin is a saturated adsorption amount of 29.8mg / g wet resin, and The best elution process the elution of 30% ethanol of 11 times the column volume (BV), and 50% ethanol of 5 times the column volume (BV ) as eluent. Purified from the total flavonoids purity of 76.63%, the extraction rate of 72%. The performance of the S-8 type resin regeneration. The total flavonoids of residual organic solvents in the S-8 resin and purified. The results show that the S-8 type resin pretreated basic residual organic solvents; the purified total flavonoids detect less than organic solvent residue. Show that the pretreatment method is effective and feasible, can be used for the purification of the total flavonoids treated resin. Mulberry hypoglycemic capsules formulations molding process. Moisture percentage as an indicator the to three thinner drug hygroscopicity results show that the hydroxypropyl cellulose can reduce the moisture absorption of the drug. The through the Orthogonal best prescription for: Drugs: thinner = 1:1 wetting agent for 70% ethanol, the amount of 12% (12mL/100g powder). Particle flow is good, without adding lubricant directly filling the 1st capsule. Mulberry hypoglycemic capsule quality standards. The mulberry leaves Biological total flavonoids from mulberry leaves, mulberry leaf alkaloids using thin layer chromatography and high performance liquid chromatography for qualitative and quantitative analysis; Each capsule total flavonoids rutin which shall be not less than 10mg of preparation the alkali to 1 - Deoxynojirimycin, which shall be not less than 3mg. Mulberry hypoglycemic capsules preliminary stability. Impact factor and accelerated test preparation Appearance, content and disintegration time changes were investigated. The results show that the temperature and light on the preparations, humidity greater impact on preparations, preparations should be sealed, cool and dry place storage. Mulberry hypoglycemic capsules preliminary pharmacodynamic study. The results show that the Mulberry hypoglycemic capsules can reduce the blood sugar of alloxan-induced diabetic mice, promote glycogen synthesis, increased liver glycogen, enhance the storage capacity of the sugar of the diabetic animals.
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