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Study on the Nanoparticles Probe Analysis by Chemiluminescence and Resonance Light Scattering Technique

Author: JiaHongXia
Tutor: LiZhengPing
School: Hebei University
Course: Analytical Chemistry
Keywords: Gold nanoparticles Silver nanoparticles DNA hybridization Chemiluminescence Resonance Light Scattering
CLC: O657.3
Type: Master's thesis
Year: 2007
Downloads: 30
Quote: 0
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Abstract


The nucleic acid is an important life macromolecules, the analysis of nucleic acids in life science research has important significance . Especially DNA (Deoxyribonucleic Acid, DNA), it is the primary genetic material , with important biological functions . Special nucleic acid sequences and single nucleotide polymorphism (SNP) detection , has a very important role in clinical diagnosis , pathological analysis , as well as food and drug detection , environmental monitoring . Therefore, the development of high selectivity , high sensitivity , fast and convenient detection of DNA hybridization method is becoming more and more urgent . In recent years metal nano- particles in the field of molecular biology research and attracted widespread attention . Metal nanoparticles its unique spectral properties and biological compatibility should be shipped in the analysis of proteins, nucleic acids and other macromolecules of life . In this article, we labeled oligonucleotide silver nanoparticles for the quantitative determination of silver nanoparticles of a labeled oligonucleotide probe chemiluminescence detection method . The method is simple , high sensitivity , quantitative detection of labeled oligonucleotide silver nanoparticles , the linear range of 5 × 10-91 × 10-g/mL detection limit (3σ) up to 2.0 × 10 -9 g / mL, further DNA hybridization analysis has laid the foundation . We also use the resonance light scattering technique to study the adsorption of single-stranded DNA (ss-DNA) and double-stranded DNA (ds-DNA) gold nanoparticles . Have different electrostatic properties found that ss-DNA and ds-DNA : ss-DNA can be directly adsorbed on citrate coated gold particles , and can enhance the stability of the gold particles , to prevent it from aggregation in a salt solution ; ds-DNA can not adsorb to the gold particles , can not prevent the aggregation of the Au nanoparticles in the salt solution . Based on this phenomenon , we have established a simple and efficient resonance light scattering method using hybrid detection of specific DNA sequences . The draw resonance light scattering intensity and the concentration of target DNA in 2 nmol / L ~~ 30 nmol / L range a good linear relationship with a detection limit of 0.8 nmol / L . And by controlling the denaturation temperature , can distinguish single-base mismatch in the target sequence exactly matches the target sequence , and opened up a new way for single nucleotide polymorphism (SNP) .

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CLC: > Mathematical sciences and chemical > Chemistry > Analytical Chemistry > Instrument analysis ( physics and physical chemistry ) > Photochemical analysis ( spectral analysis method)
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