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Effect of the Expression of StAR mRNA after Treatment of Ractopamine in Testis of Adult Rat

Author: RenXin
Tutor: ZhuWeiJie
School: Jinan University
Course: Developmental Biology
Keywords: Ractopamine (RAC) Rat Testis Steroidogenic Acute Regulatory Protein (StAR) mRNA
CLC: S859.8
Type: Master's thesis
Year: 2009
Downloads: 1
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Abstract


ObjectiveThe present study was to investigate the effect of ractopamine (RAC) on testicular gene StAR mRNA expression level in adult rats, so as to elucidate the mechanism of the StAR gene caused by RAC in testis.Animals and groupsTwenty-eight adult male Sprague-Dawley rats were randomly divided into 7 groups:N, O, P, Q; R, S and T. RAC dissolved in normal saline was given at the dose of 0,200,20 or 2 ppm in each group by intragastric administration consecutively. O-Q groups received a 1-d withdrawal period after treatment with RAC for 1 day, the others received a 14-d withdrawal period.One hundred and thirty adult male Sprague-Dawley rats were randomly assigned to 26 groups:A-M; A’-M’. RAC dissolved in normal saline was given at the dose of 0,0.2,0.02,2, or 200 ppm in each group by intragastric administration. A-C and A’-C groups received a 21-d withdrawal period after treatment with RAC consecutively for 14 days; E-G groups received a 14-d withdrawal period after treatment with RAC consecutively for 14 days; E’-G’, H-J and H’-J’groups received a 7-d withdrawal period after treatment with RAC consecutively for 14 days; K-M and K’-M’groups received a 1-d withdrawal period after treatment with RAC consecutively for 14 days MethodsIn all RAC groups, body, testis were all weighted; The expression levels of testicular steroidogenic acute regulatory (StAR) gene mRNA was determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR).All data were expressed as mean as±SD((?)+s). Statistical analysis was performed with one-way analysis of variance (ANOVA), whenever the F value was significant, the data were analysed by Duncan’s multiple comparison test to find the within-group significance at the P<0.05 level.Results1. The result of the rat body weights and testis wet weightsThe body weights of O and R groups (200 mg/kg·day) increased than that of controls (P<0.05), the remaining changes in body weights of rats in each group no significant difference (P>0.05); The body weights of I, L groups (0.2 mg/kg-day) and J, M groups (0.02 mg/kg-day) increased than that of controls (P>0.05), the others increased significantly (P＜0.05); The testis wet weights in all treatment groups decreased than that of controls, but no significant difference (P>0.05).2. The expression of testicular gene StAR mRNAThe expression levels of StAR mRNA in the testis of rats were not significant between the O-T, J’groups and controls (P>0.05); and the other groups decreased significantly (P＜0.05).The expression levels of StAR mRNA in the testis of rats of O-T groups and the dose of RAC was negatively correlated (rs=-0.475, P＜0.05), there was not a linear regression.The expression levels of StAR mRNA in the testis of rats of A-M or A’-M’groups and the dose of RAC was negatively correlated (rs=-0.476, P＜0.01) and there was a linear regression between the expression levels of StAR gene of mRNA in the testis of rats and the dose of RAC (b=-0.001, P＜0.05); The negative relationship between the dose of RAC and the withdrawal period after treatment with RAC was also observed(rs=-0.229, P＜0.05). Conclusions1. RAC could increase the body weight of adult male rats, but little effect on testicular weight. RAC could prompt muscle protein synthesis in adult male rats, thus increasing body weight.2. The expression of testicular gene StAR mRNA in groups of O-T decreased than that of controls after treatment of RAC for 1 day (P>0.05), but the expression of StAR mRNA in the testis of rats after treatment of RAC for 14 days (except for J’group) were significantly lower than that of controls (P＜0.05). There was a treatment-time-related effect on the affinity of the beta-adrenoceptors at any of the RAC doses tested on the expression of StAR mRNA in rat testis, at the same time, The linear regression relationship between the incidence of RAC and the expression of StAR mRNA in the testis of rats was also observed. Prompted a short-term RAC(14 d) exposure could reduce the expression levels of StAR mRNA in the testis of rats significantly and reduce the synthesis of steroid hormones, thereby affecting the normal functions of Sertoli cells and germ cells in the testis of rats.

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