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Primary Screening for Molecular Markers and the Regulation of Nutrition and Metabolic in Heat-resistant of Dairy

Author: ZhangJian
Tutor: ShaoTao；JiangYongQing
School: Nanjing Agricultural College
Course: Animal Nutrition and Feed Science
Keywords: Heat stress High energy feed Complex Additive Molecular marker RAPD
CLC: S858.23
Type: Master's thesis
Year: 2009
Downloads: 30
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Abstract


Heat-stress has a huge negative effect on production and reproduction performance of dairy cattle. Explore to ease the heat-stress through the aspects of nutrition and metabolism, while RAPD technology was used to select heat resistance molecular markers on dairy cattle.1.The experiment 1 was conducted to study the effects and mechanism of high energy feed on biochemical physiologic indices and production performance under heat-stress. Thirty three lactating cows were used in the experiment and divided into three groups randomly with 11 cows in each group. The cows in control group were fed with basic diets, the treatmentⅠwas supplemented with roast soybean, and treatmentⅡwas supplemented with rapeseed. The results showed that the milk yield of treatmentⅠhad significant (P<0.05) increase of 3.27% compared with control, milk yield of treatmentⅠandⅡboth increased by 2.21% and 7.31% respectively compared with previous. However, the control group had slight declining. The breath frequency of treatmentfⅠhad significantly (P<0.05) increased compared with that of the control group, and the two treatments had significant higher heartbeat times (P<0.05) compared with control.Both of treatedⅠandⅡhad an significant (P<0.05) increase in the contents of CAT,MDA and K+, but an significant decrease in contents of TP and Cl- compare with the control. TreatedⅠsignificantly(P<0.05)increased the BUN content. Cortical increased significantly (P<0.05) in the treatmentⅡas compared with control.2.The experiment 2 was conducted to study the effect of complex additive on milk performance and biochemical physiology parameter of cows under heat-stress and its mechanism. Twenty two lactating cows were divided randomly two groups with 11 cows in every group. The cows in control group were fed with basic diets; the treatment was supplemented with complex additive. The results showed that the milk yield of control had decreased 16.36% compare with before experiment, however, that of treatment had increased 1.45%. The complex additive had no effect on the milk composition, except fat content with significant decrease(P<0.05); The recta temperature of treatment had significantly (P<0.05) decreased,and the breath frequency had significantly (P<0.05) increased compare with control. Compared with the control,T3、Cor、INS、IL-2、TP、CK、SOD and LgG trended to increase(P>0.05), however, the content of T4 and cl- had an significant (P<0.05) increase. Compared with the control group, the content of PRL、LDH、MDA、K+ and Na+ trended to decrease (P>0.05), but the content of CAT had an significant (P<0.05) decrease.3.In this study,22 cows were selected from 52 cows based on average daily milk yield under heat-stress. Then select dairy heat-resistance molecular markers using RAPD. The effects of different enzyme and cycles on RAPD were analyzed. An optimization RAPD system was foud.26 primers with better effect were selected in 170 random primers using PCR-RAPD.Considered the detected result of biochemical and physiology parameters, two molecular markers relative to heat-tolerance in Holstein Cattle were selected finally. There were significantly positive correlations (P<0.05)between milk fat percentage and different fragments of primer S275 and S2011; the content of K+ and milk protein rate (P<0.05)with different fragment of primer S275 was a significantly negative correlations;T3、T4 (P<0.05)and milk yield (P<0.01)with different fragment of primer S275 and S2011 were a significant negative correlations; the content of CK with different fragment of primer S275 was a significantly positive correlations(P<0.05);There were a significantly negative correlations (P<0.05)between Na+ and different fragment of primer S2011.Analysis of sequencing results of specific fragment using BLAST, there were specific bands of primer S2011 at Dipeptidy-peptidase 6 downstream of 32Kb of Bos taurus chromosome 4 genomic. There were two Sequence in sequencing results of primer S275, one sequence was on MHC Class I-like Family Al downstream of 0.7Kb, and ULBP27 upstream of 10Kb; however, another sequence on NOL1/NOP2/Sun Domain Family of Bos taurus chromosome 4 genomic. It was suggested that primers S2011 and S275 could be used as molecular markers in filtration heat-resistant traits of dairy cattle from the result of BLAST.This study was conducted to study the effects and mechanism of high energy feed and complex additive on biochemical physiologic indices and production performance under heat-stress. It was suggested that two molecular markers were screened in heat-resistant using RAPD technology combined with physiological and biochemical indicators of dairy.

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CLC: > Agricultural Sciences > Livestock, animal medicine,hunting,silkworm,bee > Animal Medicine ( Veterinary Medicine) > Livestock, poultry, wildlife diseases > Livestock > Cow
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