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Genetic Basis of Transgressive Variation of Plant Height in Rice Introgression Lines

Author: ZhouJi
Tutor: YuSiBin
School: Huazhong Agricultural University
Course: Crop biotechnology
Keywords: rice (Oryza sativa L.) chromosome segment substitution lines (CSSLs) plant height quantitative trait loci (QTLs) transgressive variation
CLC: S511
Type: Master's thesis
Year: 2009
Downloads: 18
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Abstract


Plant height is one of the most important agronomic traits and contributes to rice yield.Transgressive segregation is a phenomenon that phenotypes exhibit in segregating hybrids that are extreme or novel relative to the parental lines.In this study,several chromosome segment substitution lines based on backcrossing and marker-assisted selection from a backcross using Nipponbare（Japonica） as a donor,Zhenshan 97B（Indica） as the receptor,were used to research the genetic basis of plant height transgressive variation.The main results of the study are as follows:One of the chromosome segment substitution lines,CSSL1 is about 56cm higher than Zhenshan 97B.Through the study on CSSL1,we found the introgression segments in CSSL1 containing two plant height QTL:qPH1 and qPH8a,explained phenotypic variation of 73.8%and 17.2%,respectively.Interaction analysis showed that the two QTLs does not exist any interaction.The effects are simple additive.The two QTLs explained phenotypic variation of 91%in all,which explained the genetic basis of plant height transgressive variation in CSSL1 well.Through the fine mapping for qPH1,it was confirmed as the SD1,which encodes the GA20 oxidase.Zhenshan 97B contained the sd1 mutant led to a lack ofbioactive GAs so that represented semi-dwarf.The introgression segments came from the Nipponbare brings the plant the wild-type SD1,which encodes a functional GA20 oxidase,makes the plant higher.Through the fine mapping for qPH8a,the region containing qPH8 was narrowed to about 278kb between marker PSB99 and RM5556.The introgression from Nipponbare containing qPH8a increased the plant height.Through the study on chromosome segment substitution lines of CSSL2,CSSL3 and CSSL4,the results showed the introgression segments lower the plant height at 0.01 significant in t test.But in the F2 group hybrid with Zhenshan97B,the plant height did not appear a clear separation.So we consider these chromosome segment substitution lines carrying minor plant height QTLs that makes them lower.Chromosome segment substitution lines which containing the SLR,OsGA2ox4, OsGA3ox2 and OsGA20ox1 alleles from Nipponbare are hybridized with CSSL5, respectively,which containing the SD1 allele from Nipponbare,to analyse the interaction between them.The study on the F2 generation showed no interaction between SD1 and the others,except the OsGA2Oox1.The chi-square test showed the F2 segregation ratio of plant height fits 13:3 which do not fit 3:1.This result suggested an interaction between SD1 and OsGA20ox1.Through a cross between Nipponbare and CSSL5,which containing the SD1 allele from Nipponbare,we gained a F2 separating population with homozygous SD1 allele.A QTL mapping was take on the F2 population.The results of the QTL mapping listed four plant height QTLs.Two of them（qPH7-3 & qPH8-3） showed positive additive effects which means the alleles from Nipponbare make the plant lower,explained phenotypic variation of 8.5%and 10%,respectively.There are also two QTLs（qPH7-2 & qPH8-2） showed negative additive effects which means the alleles from Nipponbare makes the plant higher,explained phenotypic variation of 20.3%and 8.35%,respectively. Interaction analysis discover a interaction between qPH7-3 and qPH8-2,said the effect of qPH7-3 can be increased significantly when qPH8-2 is homozygous for the allele of Nipponbare to reduce plant height.

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