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Studies on Agronomic Traits of Two Ipt-bar Transgenic Lines and the Gene-deletor Transformed into Restorer Lines of Rice

Author: JiangJie
Tutor: ZhaoDeGang
School: Guizhou University
Course: Biochemistry and Molecular Biology
Keywords: Transgenic rice Agronomic traits Exogenous gene Clear technology Agrobacterium-mediated genetic transformation
CLC: S511
Type: Master's thesis
Year: 2009
Downloads: 17
Quote: 0
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Abstract


The research on the bivalent gene transfer ipt-bar the EY105-T19 rice lines and R527-T1 rice lines under confinement , the field comparison test , at the same time , to indica rice restorer line varieties R527 and Mianhui 047 for materials , the outer removal system of gene expression vectors by Agrobacterium -mediated genetic transformation research . The results were as follows : . Transferred to the field conditions ipt-bar the bivalent genes EY105-T19 R527-T1 lines T3 population strains T6 and turn ipt-bar bivalent genes materials , their main agronomic traits the results show that , under field conditions , transferrin ipt-bar bivalent genes EY105-T19 plant height was significantly higher than the control , turn ipt-bar bivalent genes R527-T1 plant height was significantly lower than the control . Two stable transgenic lines compared with the control effective panicles , panicle length , total panicles , seed setting rate and grain weight and other agronomic traits did not differ significantly . Randomized block comparison test to prove , turn ipt - bar bivalent genes EY105 - T19 yield compared with non-transgenic varieties to improve 6.95 percent , reached a significant level , and turn ipt-bar bivalent genes R527 -T1 yield its non- transfected gene control without significant difference. Rice mature seeds the Iduced callus found to remove 75% of the endosperm reduce the initial contamination callus induction , the tested materials R527 contamination rate decreased 4.4 % , lower contamination rate Mianhui 047 6.6%. With a concentration of 2.0mg · L-1 and 3.0mg · L 2,4-D the R527 and Mianhui , 047 callus induction effect can achieve the best . In the process of tissue differentiation adventitious buds , callus using the absorbent paper dehydrated for 2-3 days adventitious buds differentiation can effectively accelerate and increase the rate of adventitious bud differentiation . Following the generation of medium Add 15g · L-1 sorbitol significantly promote embryogenic callus formation . The regenerated seedlings the Mianhui 047 53 . Agrobacterium-mediated exogenous gene removal system genetic transformation R527 found Agrobacterium concentration OD value of 0.4 infestation callus phenolic compound acetosyringone (AS) 100μmol · L is added to the total medium -1 better . 34 regenerated plants obtained two positive plants , GUS detection .

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CLC: > Agricultural Sciences > Crop > Cereal crops > Rice
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